Difference between revisions of "CI 10305, Picric Acid, LC"

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(Analytical instrumentation and procedures)
(Analytical instrumentation and procedures)
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== Analytical instrumentation and procedures ==
 
== Analytical instrumentation and procedures ==
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'''HPLC system''': The dye analyses were performed in a Thermofinnigan Surveyor HPLC-DAD system with a Thermofinnigan Surveyor PDA 5 diode array detector (Thermofinnigan, USA), an autosampler and a pump.
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'''HPLC Column''': The separations were performed in Zorbax Eclipse Plus C18 (Agilent, USA) with 5μm particle size column (2.1 mm x 150 mm). The column was kept at controlled temperature (35ºC). The samples were injected onto the column via a Rheodyne injector with a 25μl loop.
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'''LC program''': A solvent gradient of (A) TEA pH= 6,4 3mM and (B) pure methanolwas used at a flow rate of 0.3 mL/min; 0-0,01min A:B (95:5) isocratic, 5 min A:B (90:10) isocratic, 10 min A:B (70:30) isocratic, 15 min A:B (50:50) isocratic, 30 min A:B (45:55) isocratic, 35 min A:B (30:70) isocratic, 45-55 min A:B (5:95) linear, 60-70 min A:B (95:5) linear.
  
 
[1]
 
[1]

Revision as of 14:54, 3 October 2017

Usage History

Picric acid

Synonyms

C.I. 10305; 2,4,6-trinitrophenol; 1,3,5-Trinitrophenol.

Molecular Information

Picric acid Hazards

Molecular Formula C6H3N3O7

Molecular Weight 229.1 g/mol

Picric acid.png

Analytical instrumentation and procedures

HPLC system: The dye analyses were performed in a Thermofinnigan Surveyor HPLC-DAD system with a Thermofinnigan Surveyor PDA 5 diode array detector (Thermofinnigan, USA), an autosampler and a pump.

HPLC Column: The separations were performed in Zorbax Eclipse Plus C18 (Agilent, USA) with 5μm particle size column (2.1 mm x 150 mm). The column was kept at controlled temperature (35ºC). The samples were injected onto the column via a Rheodyne injector with a 25μl loop.

LC program: A solvent gradient of (A) TEA pH= 6,4 3mM and (B) pure methanolwas used at a flow rate of 0.3 mL/min; 0-0,01min A:B (95:5) isocratic, 5 min A:B (90:10) isocratic, 10 min A:B (70:30) isocratic, 15 min A:B (50:50) isocratic, 30 min A:B (45:55) isocratic, 35 min A:B (30:70) isocratic, 45-55 min A:B (5:95) linear, 60-70 min A:B (95:5) linear.

[1]

Chromatograms

Absorbance at 347nm (mAU)[1]


Results

compounds identified

References

[1] Cátia Susana da Costa Nogueira Souto, "Analysis of Early Synthetic Dyes with HPLC-DAD-MS An important database for analysis of colorants used in cultural heritage", Thesis, Master Degree in Conservation and Restoration of Textiles. UNIVERSIDADE NOVA DE LISBOA, 2010.