Difference between revisions of "Dichlorofluorescein"
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4,5-dichlorofluorescein; D&C Orange no.8 (delisted); DCF; 4,5-dichloro-3,6-fluorandiol; fluorescein chloride | 4,5-dichlorofluorescein; D&C Orange no.8 (delisted); DCF; 4,5-dichloro-3,6-fluorandiol; fluorescein chloride | ||
| − | == | + | ==Physical and Chemical Properties== |
| − | Soluble in ethanol and dilute alkali. Slightly soluble in glycols and glycerol. Insoluble in water, dilute acids, oils, fats and waxes. | + | * Soluble in ethanol and dilute alkali. Slightly soluble in glycols and glycerol. Insoluble in water, dilute acids, oils, fats and waxes. |
| − | + | * Maximum absorption wavelength=513 nm; | |
| − | Maximum absorption wavelength=513 nm; Maximum emission wavelength= 532 nm. | + | * Maximum emission wavelength= 532 nm. |
| − | + | * The fluorescence of dichlorofluorescein changes with pH. It is colorless below pH=4.0 and green fluorescent above 5.0. | |
| − | The fluorescence of dichlorofluorescein changes with pH. It is colorless below pH=4.0 and green fluorescent above 5.0. | ||
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| − | == | + | ==Resources and Citations== |
| − | |||
| − | |||
| − | + | * R. Wolbers, N. Sterman, C. Stavroudis, "Notes for Workshop on New Methods in the Cleaning of Paintings", Getty Conservation Institute, Los Angeles, 1990. | |
| − | + | * John M.Messinger "Ultraviolet-Fuorescence Microscopy of Paint Cross Sections" ''JAIC'' 31(3):267-74, 1992 | |
* Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, ''Notes for Workshop on New Methods in the Cleaning of Paintings'', J.Paul Getty Trust, Los Angeles, 1990 | * Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, ''Notes for Workshop on New Methods in the Cleaning of Paintings'', J.Paul Getty Trust, Los Angeles, 1990 | ||
Revision as of 10:41, 21 July 2022
Description
An orange reagent used as a fluorescent indicator for the presence of oils and fats. Dichlorofluorescein (DCF) is available as a powder or a premixed solution of 0.2% in ethanol. The lipid environment can effect the fluorescence color; DCF fluoresces pink in saturated lipids and bright yellow in unsaturated lipids (Wolbers et al., 1990). Acrylics can give a positive result.
Synonyms and Related Terms
4,5-dichlorofluorescein; D&C Orange no.8 (delisted); DCF; 4,5-dichloro-3,6-fluorandiol; fluorescein chloride
Physical and Chemical Properties
- Soluble in ethanol and dilute alkali. Slightly soluble in glycols and glycerol. Insoluble in water, dilute acids, oils, fats and waxes.
- Maximum absorption wavelength=513 nm;
- Maximum emission wavelength= 532 nm.
- The fluorescence of dichlorofluorescein changes with pH. It is colorless below pH=4.0 and green fluorescent above 5.0.
| Composition | C20H10Cl2O5 |
|---|---|
| CAS | 2320-96-9 |
| Molecular Weight | mol. wt. = 401.19 |
Resources and Citations
- R. Wolbers, N. Sterman, C. Stavroudis, "Notes for Workshop on New Methods in the Cleaning of Paintings", Getty Conservation Institute, Los Angeles, 1990.
- John M.Messinger "Ultraviolet-Fuorescence Microscopy of Paint Cross Sections" JAIC 31(3):267-74, 1992
- Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990
- The Merck Index, Susan Budavari (ed.), Merck Research Labs, Whitehouse Station, NJ, 12th Edition, 1996 Comment: entry 3051; CAS=2320-96-9
- CRC Handbook of Chemistry and Physics, Robert Weast (ed.), CRC Press, Boca Raton, Florida, v. 61, 1980 Comment: The fluorescence of dichlorofluorescein changes with pH. It is colorless below pH=4.0 and green fluorescent above 5.0.
- Sigma Dyes, Stains and Natural Pigments, Infrared Library, Nicolet, 1991-1995 Comment: OMNIC: formula= C20H10Cl2O5, CAS= 76-54-0