Chinese grass (Miscanthus tinctorius) LC

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Chinese grass from Tanakanao/ Photo by X. Zhang

Contents

Description

Chinese grass (Miscanthus tinctorius) is also called kariyasu, yama kariyasu (mountain kariyasu) or kizome gusa (graa for making yellow) in Japanese.

Chinese grass is a tufted perennial grass with culms 80-100 cm long.

Historical importance

Summary of results

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.

Chromatograms

Absorbance at 350nm (mAU)

Sample information

Sample information, by R. Laursen, Boston University

Identified compounds

HPLC-DAD

Larkspur.JPG

Rutin UV-Vis

Rutin.JPG

Quercetin UV-Vis

Isojpeg.jpg


Compound RT (min.) MW UV/vis Other
rutin 27.0 610 278,352 Comments here
quercetin 34.6 302 280,395,370
kaempferol 39.0 286 280,395,368
isorhamnetin 39.0 316 280,395,368

References

[1] [2] [3]

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