Dyer's greenweed (Genista tinctoria) LC

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Plants of Genista tinctoria, By H. Zell - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=10212397



The Genista tinctoria is called dyer’s greenweed or dyer's broom. It is a variable deciduous shrub growing to 60–90 centimetres (24–35 in) tall by 100 cm (39 in) wide, the stems woody, slightly hairy, and branched. Golden yellow pea-like flowers are borne in erect narrow racemes from spring to early summer.

Historical importance

Summary of results

Falvonoids were identified from the extraction of Genista tinctoria dyed samples. Luteolin and Genestin could possibly be the main dyeing components.

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.


Absorbance at 350nm (mAU)

Sample information

sample information, By R. A. Laursen, Boston University

Identified compounds

Luteolin UV-Vis


Genistein UV-Vis


Compound RT (min.) MW UV/vis Other
Luteolin 36.1 286 348 Comments here
Genistein 38.7 270 338


[1] [2] [3]

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