High-performance liquid chromatography uses a liquid mobile phase to transport and help separate the components of a mixture. The mixture is injected into a column packed with a stationary phase under high pressure. In the column, the mixture is resolved, via absorption and desorption, into its constituents. The interaction of the solute with mobile and stationary phases is dependent upon the solvent gradient and stationary phase. High-performance liquid chromatography is useful in the separation of compounds that are too nonvolatile to be separated by gas chromatography. In the cultural heritage area, high-performance liquid chromatography is for instance used for the identification of organic dyes.
high performance liquid chromatography, chromatographie en phase liquide à haute performance (Fr.)