Persian berries LC

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Persian berries,Photo by X. Zhang



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Synonyms and Related Terms

Japanese pagoda tree (Sophora japonica; Styphnolobium japonicum); waifa; enjo (Jap.)

Persia berry dyed wool sample, photo from

Summary of results

Mauris erat ligula, vestibulum id aliquam a, tempus nec mi. Cras cursus magna id justo convallis vel tincidunt augue varius. Curabitur eu ligula dui. Mauris aliquet pretium felis, nec consectetur nunc lobortis eget. Etiam cursus sem ac elit lobortis in vulputate orci mattis.

Sample preparation

Pagoda tree buds sample (~0.1g) from Tanakano, Japan was extracted with 1 mL methanol:H2O (v:v=1:1). Then the upper 30 μl of solution was removed for HPLC-DAD-MS analysis (20 μl was injected).

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.



Absorbance at 350nm (mAU)


compounds identified

Identified compounds


Pagoda tree buds.png

Rutin UV-Vis


Compound RT (min.) MW UV/vis Other
rutin 27.0 610 278,352 Comments here
quercetin 34.6 302 280,395,370
kaempferol 39.0 286 280,395,368
isorhamnetin 39.0 316 280,395,368


  • Zhang, X., and Laursen, R. A., Development of Mild Extraction Methods for the Analysis of Natural Dyes in Textiles of Historical Interest Using LC-Diode Array Detector-MS. Analytical Chemistry 77, 2022-2025 (2005).
  • Zhang, X., Corrigan, K., MacLaren, B., Leveque, M., and Laursen, R. A., Characterization of Yellow Dyes in Nineteenth Century Chinese Textiles. Studies in Conservation 52, 211-220 (2007).
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