Triphenyltetrazolium chloride

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Colorless needle crystals that yellow when exposed to light. Triphenyltetrazolium chloride (TTC) is primarily used as an analytical reagent for the detection of reducing sugars in plant and animal tissues. The colorless TTC reacts with sugars to become a deep red, water-insoluble compound (triphenylformazan). Reducing agents (i.e. borohydride, bleaching powder, chloramine T) give a positive red result.

Synonyms and Related Terms

TTC; TPTZ; red tetrazolium; tetrazolium chloride; 2,3,5-triphenyl-2H-tetrazolium chloride; Vitastain; tetrazole red

Chemical structure

Triphenyltetrazolium chloride.jpg

Other Properties

Soluble in ethanol, water, acetone. Insoluble in ether.

Composition C19H15ClN4
CAS 298-96-4
Melting Point 243 (dec)
Molecular Weight mol. wt. = 334.5848

Hazards and Safety

Flammable. Flash point = 40C

Contact causes irritation.

Fisher Scientific: MSDS

Sources Checked for Data in Record

  • Richard S. Lewis, Hawley's Condensed Chemical Dictionary, Van Nostrand Reinhold, New York, 10th ed., 1993
  • Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990
  • The Merck Index, Martha Windholz (ed.), Merck Research Labs, Rahway NJ, 10th edition, 1983 Comment: entry 9874
  • Aldrich Chemical Catalog Comment: p. 1502