Weld LC

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Contents

Description

. Please note - this page is currently a draft - the slider gallery images are not for Weld.

Summary of results

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Sample preparation

Weld (Reseda Luteola L)leaves (~0.1g) from Kremer were extracted with 1 mL methanol:H2O (v:v=1:1). Then the upper 30 uL of solution was removed for HPLC-DAD-MS analysis (20 uL was injected).

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.


Chromatograms

HPLC-DAD

weld extract, Absorbance at 350nm (mAU)

Result

compounds identified

Identified compounds

HPLC-DAD

Weld.JPG

Rutin UV-Vis

Rutin.JPG

Quercetin UV-Vis

Quercetin.jpg

MS-TIC(Neg)

F441sem.jpg

EDS

F441edsbw.jpg

Chemical structure

Indigo.jpg


Compound RT (min.) MW UV/vis Other
Luteolin 7-glucoside 28.0 448 278,348 Comments here
Luteolin 36.0 286 280,348
Apigenin 39.5 270 280,338
Chryseriol 39.5 300 280,395,338

References

  • Zhang, X., and Laursen, R. A., Development of Mild Extraction Methods for the Analysis of Natural Dyes in Textiles of Historical Interest Using LC-Diode Array Detector-MS. Analytical Chemistry 77, 2022-2025 (2005).
  • Zhang, X., Corrigan, K., MacLaren, B., Leveque, M., and Laursen, R. A., Characterization of Yellow Dyes in Nineteenth Century Chinese Textiles. Studies in Conservation 52, 211-220 (2007).
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