Grape leaves (Vitis vinifera) LC

cultivated Common Grape Vine, By eflon (Alex from Ithaca, NY) - Flickr, CC BY 2.0, https://commons.wikimedia.org/w/index.php?curid=16961598

Description

Vitis vinifera (common grape vine) is a species of Vitis, native to the Mediterranean region, central Europe, and southwestern Asia, from Morocco and Portugal north to southern Germany and east to northern Iran.[1] It is a liana growing to 35 yards (32 m) in length, with flaky bark. The fruit is a berry, known as a grape; in the wild species it is 6 mm (0.24 in) diameter and ripens dark purple to blackish with a pale wax bloom; in cultivated plants it is usually much larger, up to 3 cm (1.2 in) long, and can be green, red, or purple (black). The species typically occurs in humid forests and streamsides.

The leaves were used to dye yellow.

Summary of results

Flavonoid glycosides were identified, mainly quercetin 3-glucuronide as the major compound.

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the positive and negative ion mode.

Chromatograms

Absorbance at 350nm (mAU)

Sample information

sample information, By R. A. Laursen, Boston University

Identified compounds

Quercetin 3-glucuronide UV-Vis

Compound	RT (min.)	MW	UV/vis	Other
Rutin	31.7	610	354	minor peak
Quercetin 3-glucuronide	32.6	478	354	Major component
Luteolin glucoside?	34.8	448	348	Possible

References

[1] Euro+Med Plantbase Project: Vitis vinifera.