CI 10305, Picric Acid, LC

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Usage History

Metanil Yellow

Synonyms

C.I. 13065; C.I. Acid Yellow 36; C.I. Acid Yellow 36 monosodium salt; C.I. Acid Yellow 36, monosodium salt; C.I. Acid Yellow 36, monosodium salt; 3-(4-Anilinophenylazo)benzenesulfonic acid sodium salt; 3-[[4-(Phenylamino)phenyl]azo]benzenesulfonic acid monosodium salt; Acid Yellow 36; metanil yellow (C.I. 13065); sodium 3-(p-anilinophenylazo)benzenesulphonate; Acid Yellow 36 (13065); Acid Golden Yellow G; C.I.Acid Yellow 36; [3-(4-anilinophenyl)azophenyl]sulfanyloxyperoxysodium


Molecular Information

Molecular Formula C18H14N3NaO3S

Molecular Weight 375.3768

CI 13065.PNG

Analytical instrumentation and procedures

Dyes were extracted from textiles (silk or wool) using formic acid/methanol (5:95, v/v).

HPLC column: Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size).

HPLC-DAD-MS instrument: HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer.

HPLC gradient profile: Separations were done on a The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative ion mode because metanil Yellow is negatively charged without Na+.

Chromatograms

Absorbance at 450nm (mAU)[1]


Results

compounds identified

References

[1] Zhang, X. Laursen R. and Osipova S. (2005) "Analysis of dyes in some 19th-century Uzbek suzanis"; Dyes in History and Archaeology To be published.