Acridine yellow

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Absorption and fluorescence emission spectra


Yellow crystals that produce dark brown solution. Dilute solutions of acridine yellow are fluorescent. Acridine yellow is used as a Fluorochrome for biological staining. It has a mean excitation wavelength of 470 nm (blue) and a mean emission wavelength of 550 nm (Wolbers et al., 1990).

Synonyms and Related Terms

CI 46025; 3,6-diamino-2,7-dimethylacridine hydrochloride

Chemical structure

Acridine yellow.jpg


Strongly irritating to skin and mucous membranes.

Fisher Scientific: MSDS

Physical and Chemical Properties

  • Soluble in ethanol, ether, hydrocarbons, carbon disulfide, boiling water. Insoluble in water, benzene.
  • Maximum absorption wavelength = 470 nm;
  • Maximum emission wavelength = 550 nm
Composition C13H11N3
CAS 135-49-9
Melting Point 281 C
Molecular Weight mol. wt. = 273.76

Resources and Citations

  • Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990