Berberis dye (berberis Julianae) LC

Berberis julianae bush, photo by X. Zhang at the Arnold Arboretum, Boston

Description

Berberis julianae, also called Mahonia julianae, is a flowering evergreen shrub native to Central China. It is widely grown as an ornamental in other temperate regions. It grows to 6' to 8' tall and 4' to 6' wide.

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.

Chromatograms

Absorbance at 350nm (mAU), By X. Zhang

sample information

Identified compounds

Compound	RT (min.)	MW	UV/vis	Other
Jatrorrhizine	29.4	337	344,426
Berberine	32.7	335	344,426	co-elutes with palmatine
Palmatine	32.7	351	344,426	co-elutes with berberin

References

[1] [2] [3]