Difference between revisions of "Berberis dye (berberis tibetasia) LC"

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= Chromatograms =
 
= Chromatograms =
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''berberis tibetasia''dyed silk sample (direct dye without mordant) was extracted and the extract was injected in HPLC-DAD-MS for analysis.
  
 
HPLC-DAD profile and compunds identified
 
HPLC-DAD profile and compunds identified

Revision as of 09:41, 22 June 2017

Yellow larkspur/ picture from Denver Botanic Gardens

Description

Historical importance

Summary of results

Analytical instrumentation and procedures

Chromatograms

berberis tibetasiadyed silk sample (direct dye without mordant) was extracted and the extract was injected in HPLC-DAD-MS for analysis.

HPLC-DAD profile and compunds identified

Absorbance at 350nm (mAU)

Identified compounds

HPLC-DAD

Larkspur.JPG

Rutin UV-Vis

Rutin.JPG

Quercetin UV-Vis

Isojpeg.jpg


Compound RT (min.) MW UV/vis Other
rutin 27.0 610 278,352 Comments here
quercetin 34.6 302 280,395,370
kaempferol 39.0 286 280,395,368
isorhamnetin 39.0 316 280,395,368

References

[1] [2] [3]