Difference between revisions of "Berberis dye (berberis tibetasia) LC"

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Line 34: Line 34:
 
! scope="col"| Other
 
! scope="col"| Other
 
|-
 
|-
| rutin
+
| palmatine
| 27.0
+
| 37.1
| 610
+
| 351
| 278,352
+
| 348,428
| Comments here
+
| minor component
 
|-
 
|-
| quercetin
+
| jatrorrhizine
| 34.6
+
| 34.4
| 302
+
| 337
| 280,395,370
+
| 348,428
 
|
 
|
 
|-
 
|-
| kaempferol
+
| berberine
| 39.0
+
| 37.1
| 286
+
| 335
| 280,395,368
+
| 348,428
|
+
| major component
|-
 
| isorhamnetin
 
| 39.0
 
| 316
 
| 280,395,368
 
 
|
 
|
 
|}
 
|}

Revision as of 09:43, 22 June 2017

Yellow larkspur/ picture from Denver Botanic Gardens

Description

Historical importance

Summary of results

Analytical instrumentation and procedures

Chromatograms

berberis tibetasiadyed silk sample (direct dye without mordant) was extracted and the extract was injected in HPLC-DAD-MS for analysis.

HPLC-DAD profile and compunds identified

Absorbance at 350nm (mAU)

Identified compounds

HPLC-DAD

Larkspur.JPG

Rutin UV-Vis

Rutin.JPG

Quercetin UV-Vis

Isojpeg.jpg


Compound RT (min.) MW UV/vis Other
palmatine 37.1 351 348,428 minor component
jatrorrhizine 34.4 337 348,428
berberine 37.1 335 348,428 major component

References

[1] [2] [3]