https://cameo.mfa.org/index.php?title=CI_16185,_Amaranth,_Acid_Red_27,_LC&feed=atom&action=history CI 16185, Amaranth, Acid Red 27, LC - Revision history 2024-03-28T17:43:12Z Revision history for this page on the wiki MediaWiki 1.35.3 https://cameo.mfa.org/index.php?title=CI_16185,_Amaranth,_Acid_Red_27,_LC&diff=61253&oldid=prev Zhang: /* Results */ 2017-10-23T18:06:36Z <p><span dir="auto"><span class="autocomment">Results</span></span></p> <table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 18:06, 23 October 2017</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l38" >Line 38:</td> <td colspan="2" class="diff-lineno">Line 38:</td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Results ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Results ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[<del class="diffchange diffchange-inline">FilAcid Red </del>27.JPG|center|frame|compound identified]]</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[<ins class="diffchange diffchange-inline">File:Acid red </ins>27 <ins class="diffchange diffchange-inline">result</ins>.JPG|center|frame|compound identified]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td></tr> </table> Zhang https://cameo.mfa.org/index.php?title=CI_16185,_Amaranth,_Acid_Red_27,_LC&diff=61250&oldid=prev Zhang: /* Chromatograms */ 2017-10-23T18:05:41Z <p><span dir="auto"><span class="autocomment">Chromatograms</span></span></p> <table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 18:05, 23 October 2017</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l33" >Line 33:</td> <td colspan="2" class="diff-lineno">Line 33:</td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:<del class="diffchange diffchange-inline">Fast </del>Red <del class="diffchange diffchange-inline">E LC</del>.JPG|center|frame|<del class="diffchange diffchange-inline">Fast Red E </del>Absorbance at <del class="diffchange diffchange-inline">520nm </del>(mAU) By Cátia Souto, University of Lisbon, Portugal 2010 [1]]]</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:<ins class="diffchange diffchange-inline">Acid </ins>Red <ins class="diffchange diffchange-inline">27</ins>.JPG|center|frame| <ins class="diffchange diffchange-inline">Amaranth </ins>Absorbance at <ins class="diffchange diffchange-inline">525 nm </ins>(mAU) By Cátia Souto, University of Lisbon, Portugal 2010 [1]]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Results ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Results ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[<del class="diffchange diffchange-inline">File:Fast </del>Red <del class="diffchange diffchange-inline">E result</del>.JPG|center|frame|<del class="diffchange diffchange-inline">compounds </del>identified]]</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[<ins class="diffchange diffchange-inline">FilAcid </ins>Red <ins class="diffchange diffchange-inline">27</ins>.JPG|center|frame|<ins class="diffchange diffchange-inline">compound </ins>identified]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td></tr> </table> Zhang https://cameo.mfa.org/index.php?title=CI_16185,_Amaranth,_Acid_Red_27,_LC&diff=61214&oldid=prev Zhang: /* Molecular Information */ 2017-10-23T17:40:17Z <p><span dir="auto"><span class="autocomment">Molecular Information</span></span></p> <table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 17:40, 23 October 2017</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l13" >Line 13:</td> <td colspan="2" class="diff-lineno">Line 13:</td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Molecular Weight 604.473 g/mol</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Molecular Weight 604.473 g/mol</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:<del class="diffchange diffchange-inline">Fast </del>red <del class="diffchange diffchange-inline">E </del>structure.JPG|center|frame|<del class="diffchange diffchange-inline">Orange G</del>, Acid <del class="diffchange diffchange-inline">Orange 2</del>]]</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:<ins class="diffchange diffchange-inline">Acid </ins>red <ins class="diffchange diffchange-inline">27 </ins>structure.JPG|center|frame|<ins class="diffchange diffchange-inline">Amaranth</ins>, Acid <ins class="diffchange diffchange-inline">Red 27</ins>]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Analytical instrumentation and procedures ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Analytical instrumentation and procedures ==</div></td></tr> </table> Zhang https://cameo.mfa.org/index.php?title=CI_16185,_Amaranth,_Acid_Red_27,_LC&diff=61183&oldid=prev Zhang: /* Synonyms */ 2017-10-23T17:26:02Z <p><span dir="auto"><span class="autocomment">Synonyms</span></span></p> <table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 17:26, 23 October 2017</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l5" >Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Synonyms ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Synonyms ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>C.I. 16185. Amaranth; Acid Red 27;</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>C.I. 16185. Amaranth; Acid Red 27; <ins class="diffchange diffchange-inline">Azorubin S, FD &amp; C Red Dye No. 2.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Molecular Information ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Molecular Information ==</div></td></tr> </table> Zhang https://cameo.mfa.org/index.php?title=CI_16185,_Amaranth,_Acid_Red_27,_LC&diff=61182&oldid=prev Zhang: /* Usage History */ 2017-10-23T17:23:47Z <p><span dir="auto"><span class="autocomment">Usage History</span></span></p> <table class="diff diff-contentalign-left diff-editfont-monospace" data-mw="interface"> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 17:23, 23 October 2017</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l1" >Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Usage History ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Usage History ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'>−</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[Amaranth, Acid Red 27]</div></td><td class='diff-marker'>+</td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[<ins class="diffchange diffchange-inline">https://en.wikipedia.org/wiki/Amaranth_(dye) </ins>Amaranth, Acid Red 27]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr> <tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Synonyms ==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Synonyms ==</div></td></tr> </table> Zhang https://cameo.mfa.org/index.php?title=CI_16185,_Amaranth,_Acid_Red_27,_LC&diff=61181&oldid=prev Zhang: Created page with "== Usage History == [Amaranth, Acid Red 27] == Synonyms == C.I. 16185. Amaranth; Acid Red 27; == Molecular Information == Molecular Formula C20H11N2Na3O10S3 Molecular W..." 2017-10-23T17:23:18Z <p>Created page with &quot;== Usage History == [Amaranth, Acid Red 27] == Synonyms == C.I. 16185. Amaranth; Acid Red 27; == Molecular Information == Molecular Formula C20H11N2Na3O10S3 Molecular W...&quot;</p> <p><b>New page</b></p><div>== Usage History ==<br /> <br /> [Amaranth, Acid Red 27]<br /> <br /> == Synonyms ==<br /> <br /> C.I. 16185. Amaranth; Acid Red 27;<br /> <br /> == Molecular Information ==<br /> <br /> Molecular Formula C20H11N2Na3O10S3<br /> <br /> Molecular Weight 604.473 g/mol<br /> <br /> [[File:Fast red E structure.JPG|center|frame|Orange G, Acid Orange 2]]<br /> <br /> == Analytical instrumentation and procedures ==<br /> <br /> '''Extraction''': The extraction was carried out as follows: a small sample of thread was extracted with the TFA 2M in 1.5ml eppendorfs for 30 min, in 60ºC water bath, with constant agitation. After extraction, each extract was dried in a vacuum system, where the resulting dry residues were reconstituted with 50 μl H2O: MeOH (80:20,v/v). <br /> <br /> <br /> '''HPLC system''': The dye analyses were performed in a Thermofinnigan Surveyor HPLC-DAD system with a Thermofinnigan Surveyor PDA 5 diode array detector (Thermofinnigan, USA), an autosampler and a pump. The samples were injected onto the column via a Rheodyne injector with a 25μl loop. <br /> <br /> '''HPLC Column''': The separations were performed in Zorbax Eclipse Plus C18 (Agilent, USA) with 5μm particle size column (2.1 mm x 150 mm). The column was kept at controlled temperature (35ºC). <br /> <br /> '''LC program''': A solvent gradient of (A) TEA pH= 6,4 3mM and (B) pure methanolwas used at a flow rate of 0.3 mL/min; 0-0,01min A:B (95:5) isocratic, 5 min A:B (90:10) isocratic, 10 min A:B (70:30) isocratic, 15 min A:B (50:50) isocratic, 30 min A:B (45:55) isocratic, 35 min A:B (30:70) isocratic, 45-55 min A:B (5:95) linear, 60-70 min A:B (95:5) linear.<br /> <br /> '''LC-DAD-MSn''': The analyses of dyes were performed on a LC-MS with ProStar 410 autosampler, two 212-LC chromatography pumps, a ProStar 335 diode array detector and a 500-MS ion trap mass spectrometer with an electrospray ionization (ESI) ion source (Varian, Palo Alto, CA, USA). Data acquisition and processing were performed using Varian MS Control 6.9 software.The separations were carried out using a Zorbax Eclipse Plus (Agilent, USA) with 5 μm particle size column (2.1 mm x 150 mm). The column was kept at controlled temperature (35ºC). The samples were injected onto the column via a Rheodyne injector with a 20 μL loop. The gradient adapted from and described in 2.3.1 was used at a flow rate of 0.2 mL/min. The mass spectra were acquired in negative and positive mode depending on the structures. The optimized parameters were as follows: ion spray voltage,  4,8 kV; capillary voltage, 20 V; RF loading 80%. Nitrogen was used as nebulising and drying gas, at a pressure of 35 and 15 psi; drying gas temperature, 300ºC. The multistage MS (MSn) spectra were obtained with an isolation window of 2.0 Da, excitation energy values of 0,9 to 1,5 V and an excitation time of 10 ms.<br /> <br /> [1]<br /> <br /> = Chromatograms =<br /> <br /> <br /> [[File:Fast Red E LC.JPG|center|frame|Fast Red E Absorbance at 520nm (mAU) By Cátia Souto, University of Lisbon, Portugal 2010 [1]]]<br /> <br /> <br /> == Results ==<br /> <br /> [[File:Fast Red E result.JPG|center|frame|compounds identified]]<br /> <br /> == References ==<br /> <br /> [1] Cátia Susana da Costa Nogueira Souto, &quot;Analysis of Early Synthetic Dyes with HPLC-DAD-MS An important database for analysis of colorants used in cultural heritage&quot;, Thesis, Master Degree in Conservation and Restoration of Textiles. UNIVERSIDADE NOVA DE LISBOA, 2010. <br /> <br /> [[Category:Dye Analysis]]<br /> [[Category:Reference Materials]]<br /> [[Category:Synthetic Dyes]]</div> Zhang