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EEM 3D spectrum of Flavonoid on print by Hokusai, Actors Ichikawa Danjûrô VII as Asahina and Ichikawa Monnosuke III as Tsukisayo, MFA # 11.20417 Pt 7

Samples were analyzed with a Cary Eclipse fluorescence spectrometer (Agilent Technologies), which has a 15W xenon flash lamp source and spectra were collected for excitation wavelengths between 250-600 nm and emission wavelengths between 400-650nm at steps of 10 nm. Analyses were carried out with an external quartz fiber optic probe; WinFLR software was used for acquisition and data analysis.