Difference between revisions of "Berberis dye (berberis tibetasia) LC"

From CAMEO
Jump to navigation Jump to search
Line 35: Line 35:
 
|-
 
|-
 
| palmatine
 
| palmatine
| 37.1
+
| 32.5
 
| 351
 
| 351
 
| 348,428
 
| 348,428
Line 41: Line 41:
 
|-
 
|-
 
| jatrorrhizine
 
| jatrorrhizine
| 34.4
+
| 29.6
 
| 337
 
| 337
 
| 348,428
 
| 348,428
Line 47: Line 47:
 
|-
 
|-
 
| berberine
 
| berberine
| 37.1
+
| 32.5
 
| 335
 
| 335
 
| 348,428
 
| 348,428

Revision as of 08:44, 22 June 2017

Yellow larkspur/ picture from Denver Botanic Gardens

Description

Historical importance

Summary of results

Analytical instrumentation and procedures

Chromatograms

berberis tibetasiadyed silk sample (direct dye without mordant) was extracted and the extract was injected in HPLC-DAD-MS for analysis.

HPLC-DAD profile and compunds identified

Absorbance at 350nm (mAU)

Identified compounds

HPLC-DAD

Larkspur.JPG

Rutin UV-Vis

Rutin.JPG

Quercetin UV-Vis

Isojpeg.jpg


Compound RT (min.) MW UV/vis Other
palmatine 32.5 351 348,428 minor component
jatrorrhizine 29.6 337 348,428
berberine 32.5 335 348,428 major component

References

[1] [2] [3]