Difference between revisions of "Excitation emission matrix (EEM)"

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[[File:Excitation Emission Matrix Horiba.jpg|thumb|Exictation Emission Matrix plot from Horiba: [https://www.horiba.com/int/scientific/technologies/fluorescence-spectroscopy/what-is-an-excitation-emission-matrix-eem/ EEM]]]
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[[File:MFA EEM.jpg|thumb|EEM analysis of colorants using an Agilent Cary Eclipse Spectrometer with a fiber optic probe at the MFA lab (MFA.org)]]
 
== Description ==
 
== Description ==
The Excitation Emission Matrix (EEM) is a specific measurement that is becoming more widely used within the field of fluorescence spectroscopy. An EEM compilation is essentially a three dimensional scan that results in a contour plot of excitation wavelength vs. emission wavelength vs. fluorescence intensity.
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The Excitation Emission Matrix (EEM) is a specific measurement that is becoming more widely used within the field of fluorescence spectroscopy. In a typical fluorescence (emission) measurement, the excitation wavelength is fixed and the detection wavelength varies, while in a fluorescence excitation measurement the detection wavelength is fixed and the excitation wavelength is varied across a region of interest. An emission map is measured by recording the emission spectra resulting from a range of excitation wavelengths and combining them all together. This compilation is a three dimensional scan that results can best be represented as a contour plot of excitation wavelength vs. emission wavelength vs. fluorescence intensity.  
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[[File:Derrick Fluorescence reds max.jpg|thumb|Fluorescence excitation and emission maxima for some organic reds (MFA)]]
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[[File:MFA EEM reds on print.jpg|thumb|3-dimensional EEM spectra of pink and red colorants on a Japanese print by Harunobu ([https://collections.mfa.org/objects/481262/yoshiwara-bijin-awase-the-beautiful-women-of-the-yoshiwara?ctx=6b7a658d-e4f5-4260-bc54-d720a1d30e0d&idx=4 MFA # 2006.1537.2)]]]
  
A type of electromagnetic spectroscopy that analyzes fluorescence from a sample. Fluorescence spectroscopy involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light. A complementary technique is [[absorption spectroscopy]].
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Excitation can occur at many wavelengths. When we set the instrument to scan through several excitation wavelengths, we can determine which wavelength excites the most molecules to fluoresce.  This is called the Excitation maxima. Exposing the compound to this maximum excitation wavelength we can measure the emission curve  The point with the highest emission is called the Emission max. For these three reds shown in the plot, their Excitation and Emission maxima are distinctly different.  Additionally, the maxima that the emission for safflower is in the yellow region, madder is in the orange region, and sappanwood is in the red region.
  
In a typical fluorescence (emission) measurement, the excitation wavelength is fixed and the detection wavelength varies, while in a fluorescence excitation measurement the detection wavelength is fixed and the excitation wavelength is varied across a region of interest. An emission map is measured by recording the emission spectra resulting from a range of excitation wavelengths and combining them all together. This is a three dimensional surface data set: emission intensity as a function of excitation and emission wavelengths, and is typically depicted as a contour map.
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THE MFA Scientific lab EEM instrument is set to step through the excitation wavelengths every 10 nm and collects a full emission curve at each excitation wavelength. So Instead of looking at the single line plots, we usually plot a contour map that provides the full data in the form of a three dimensional plot were the excitation wavelength is given on the vertical axis and the emission wavelength on the horizontal access.  The intensity of the absorption or emission is given by the color with dark red being the most intense. The angled bar on the plot is where the excitation and emission wavelengths are identical, thus the detector is saturated at these points.
  
 
== Synonyms and Related Terms ==
 
== Synonyms and Related Terms ==
  
EEM; Excitation-emission matrix spectroscopy; Flourescence spectroscopy; fluorimetry; spectrofluorometry
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EEM; Excitation-emission matrix spectroscopy; Fluorescence spectroscopy; Fluorimetry; Spectrofluorimetry
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==Additional Images==
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<gallery>
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File:Excitation Emission Matrix Horiba.jpg|Excitation Emission Matrix plot from Horiba: [https://www.horiba.com/int/scientific/technologies/fluorescence-spectroscopy/what-is-an-excitation-emission-matrix-eem/ EEM]
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File:Derrick Fluorescence red comparison.jpg|3-dimensional contour plots for some organic reds; (MFA)
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File:MFA EEM yellow 3D.jpg|thumb|3-D EEM spectra for some common yellow colorants; (MFA)
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</gallery>
  
 
==Resources and Citations==
 
==Resources and Citations==

Latest revision as of 07:35, 17 November 2023

EEM analysis of colorants using an Agilent Cary Eclipse Spectrometer with a fiber optic probe at the MFA lab (MFA.org)

Description

The Excitation Emission Matrix (EEM) is a specific measurement that is becoming more widely used within the field of fluorescence spectroscopy. In a typical fluorescence (emission) measurement, the excitation wavelength is fixed and the detection wavelength varies, while in a fluorescence excitation measurement the detection wavelength is fixed and the excitation wavelength is varied across a region of interest. An emission map is measured by recording the emission spectra resulting from a range of excitation wavelengths and combining them all together. This compilation is a three dimensional scan that results can best be represented as a contour plot of excitation wavelength vs. emission wavelength vs. fluorescence intensity.

Fluorescence excitation and emission maxima for some organic reds (MFA)
3-dimensional EEM spectra of pink and red colorants on a Japanese print by Harunobu (MFA # 2006.1537.2)

Excitation can occur at many wavelengths. When we set the instrument to scan through several excitation wavelengths, we can determine which wavelength excites the most molecules to fluoresce. This is called the Excitation maxima. Exposing the compound to this maximum excitation wavelength we can measure the emission curve The point with the highest emission is called the Emission max. For these three reds shown in the plot, their Excitation and Emission maxima are distinctly different. Additionally, the maxima that the emission for safflower is in the yellow region, madder is in the orange region, and sappanwood is in the red region.

THE MFA Scientific lab EEM instrument is set to step through the excitation wavelengths every 10 nm and collects a full emission curve at each excitation wavelength. So Instead of looking at the single line plots, we usually plot a contour map that provides the full data in the form of a three dimensional plot were the excitation wavelength is given on the vertical axis and the emission wavelength on the horizontal access. The intensity of the absorption or emission is given by the color with dark red being the most intense. The angled bar on the plot is where the excitation and emission wavelengths are identical, thus the detector is saturated at these points.

Synonyms and Related Terms

EEM; Excitation-emission matrix spectroscopy; Fluorescence spectroscopy; Fluorimetry; Spectrofluorimetry

Additional Images

Resources and Citations