Difference between revisions of "Fluorescein isothiocyanate"

Latest revision as of 10:13, 25 July 2022

Description

A Fluorescent dye used to detect proteins. Fluorescein isothiocyanate (FITC) is used in biochemistry for the detection of pathogens. It has also been used to detect proteins in paint cross sections (Wolbers et al 1990). However, one study has shown that FITC can also fluoresce with Oil and acrylic media (Messinger 1992). FITC is sometimes incorrectly called Fluorescein.

Synonyms and Related Terms

FITC; 5-FITC; 2-(6-hydroxy-3-oxo-(3H)-xanthene-9-yl)-5-isothiocyanato benzoic acid

Chemical structure

Risks

Skin contact causes irritation.
Fisher Scientific: MSDS

Physical and Chemical Properties

Soluble in water, dimethyl formamide, dimethyl sulfoxide.
Maximum absorption wavelength = 490 nm
Maximum emission wavelength = 520 nm

Composition	C21H11NO5S
CAS	3326-32-7
Melting Point	>360 C
Molecular Weight	mol. wt. = 389.38

Resources and Citations

J.Messinger, "Ultraviolet-Fluorescence Microscopy of Paint Cross Sections: Cycloheptaamylose-Dansyl Chloride Complex as a Protein-Selective Stain" JAIC 31:267-74, 1992.

Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990 Comment: mean excitation wavelength = 490 nm -blue and mean emission wavelength = 525 nm

Aldrich Chemical Catalog Comment: p. 725 - max=499

Website: www.mdyn.com/application_notes/technotes - gives max abs =490 and max emission = 520

Website: pingu.salk.edu/fcm/fluo.html - gives excitation and emisson as 495, 519

Website: www.probes.com/handbook/sections - gives abs and emission as 494, 518

@@ Line 1: / Line 1: @@
 == Description ==
-A [http://cameo.mfa.org/materials/fullrecord.asp?name=fluorescent dye fluorescent dye] used to detect [http://cameo.mfa.org/materials/fullrecord.asp?name=protein proteins]. Fluorescein isothiocyanate (FITC) is used in biochemistry for the detection of pathogens. It has also been used to detect proteins in paint cross sections (Wolbers et al 1990). However, one study has shown that FITC can also fluoresce with [http://cameo.mfa.org/materials/fullrecord.asp?name=oil oil] and [http://cameo.mfa.org/materials/fullrecord.asp?name=acrylic resin acrylic] media (Messinger 1992). FITC is sometimes incorrectly called [http://cameo.mfa.org/materials/fullrecord.asp?name=fluorescein fluorescein].
+A [[fluorescent dye]] used to detect [[protein|proteins]]. Fluorescein isothiocyanate (FITC) is used in biochemistry for the detection of pathogens. It has also been used to detect proteins in paint cross sections (Wolbers et al 1990). However, one study has shown that FITC can also fluoresce with [[oil]] and [[acrylic resin|acrylic]] media (Messinger 1992). FITC is sometimes incorrectly called [[fluorescein]].
 == Synonyms and Related Terms ==
@@ Line 9: / Line 9: @@
 [[[SliderGallery rightalign|fluorescein isothiocyanate.jpg~Chemical structure]]]
-== Other Properties ==
+== Risks ==
-Soluble in water, dimethyl formamide, dimethyl sulfoxide.
+* Skin contact causes irritation.
+* Fisher Scientific: [https://fscimage.fishersci.com/msds/97111.htm MSDS]
-Maximum absorption wavelength = 490 nm
+==Physical and Chemical Properties==
-Maximum emission wavelength = 520 nm
+* Soluble in water, dimethyl formamide, dimethyl sulfoxide.
+* Maximum absorption wavelength = 490 nm
+* Maximum emission wavelength = 520 nm
 {| class="wikitable"
@@ Line 26: / Line 29: @@
 |-
 ! scope="row"| Melting Point
-| >360
+| >360 C
 |-
 ! scope="row"| Molecular Weight
@@ Line 32: / Line 35: @@
 |}
-== Hazards and Safety ==
+==Resources and Citations==
-Skin contact causes irritation.
+* J.Messinger, "Ultraviolet-Fluorescence Microscopy of Paint Cross Sections: Cycloheptaamylose-Dansyl Chloride Complex as a Protein-Selective Stain" JAIC 31:267-74, 1992.
-Fisher Scientific: [https://fscimage.fishersci.com/msds/97111.htm MSDS]
+* Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, ''Notes for Workshop on New Methods in the Cleaning of Paintings'', J.Paul Getty Trust, Los Angeles, 1990  Comment: mean excitation wavelength = 490 nm -blue and mean emission wavelength = 525 nm
-== Additional Information ==
-R. Wolbers, N. Sterman, C. Stavroudis, "Notes for Workshop on New Methods in the Cleaning of Paintings", Getty Conservation Institute, Los Angeles, 1990.  J.Messinger, "Ultraviolet-Fluorescence Microscopy of Paint Cross Sections: Cycloheptaamylose-Dansyl Chloride Complex as a Protein-Selective Stain" JAIC 31:267-74, 1992.
-== Authority ==
-* Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, ''Notes for Workshop on New Methods in the Cleaning of Paintings'', J.Paul Getty Trust, Los Angeles, 1990  Comment: mean excitation wavelength = 490 nm -blue and mean emission wavelength = 525 nm
 * Aldrich Chemical Catalog  Comment: p. 725 - max=499
-* Website address 1, Website address 1  Comment: www.mdyn.com/application_notes/technotes - gives max abs =490 and max emission = 520
+* Website: www.mdyn.com/application_notes/technotes - gives max abs =490 and max emission = 520
-* Website address 2  Comment: pingu.salk.edu/fcm/fluo.html - gives excitation and emisson as 495, 519
+* Website: pingu.salk.edu/fcm/fluo.html - gives excitation and emisson as 495, 519
-* Website address 3  Comment: www.probes.com/handbook/sections - gives abs and emission as 494, 518
+* Website: www.probes.com/handbook/sections - gives abs and emission as 494, 518
 [[Category:Materials database]]