Difference between revisions of "Thin-layer chromatography"
(username removed) |
|||
| (One intermediate revision by one other user not shown) | |||
| Line 5: | Line 5: | ||
== Synonyms and Related Terms == | == Synonyms and Related Terms == | ||
| − | TLC; thin layer chromatography; | + | TLC; thin layer chromatography; Dünnschichtchromatographie (Deut.); chromatographie en couche mince (Fr.); |
| − | == | + | ==Resources and Citations== |
| − | M.Striegel, J.Hill, Thin-Layer Chromatography for Binding Media Analysis, Getty Conservation Institute, Los Angeles, 1996. | + | * M.Striegel, J.Hill, Thin-Layer Chromatography for Binding Media Analysis, Getty Conservation Institute, Los Angeles, 1996. |
[[Category:Materials database]] | [[Category:Materials database]] | ||
Latest revision as of 12:54, 8 June 2022
Description
A method to separate and characterize components in a mixture. In thin-layer chromatography (TLC) the stationary phase is prepared as a thin layer of an absorbent coated on a plastic sheet or glass plate. A drop of the analyte mixture is placed near the lower edge of the plate then the plate is placed in a solvent bath. Capillary action carries the solvent up the plate. The solvent selectively absorbs and desorbs components in the analyte as it rises. This results in each component traveling slightly different distances in the solvent front thus giving an effective separation. Identification is made by the comparison of the unknown analyte components with known reference materials analyzed under the same conditions.
Synonyms and Related Terms
TLC; thin layer chromatography; Dünnschichtchromatographie (Deut.); chromatographie en couche mince (Fr.);
Resources and Citations
- M.Striegel, J.Hill, Thin-Layer Chromatography for Binding Media Analysis, Getty Conservation Institute, Los Angeles, 1996.