Difference between revisions of "Cocklebur (Xanthium strumarium) LC"

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== Description ==
 
== Description ==
 
+
[https://en.wikipedia.org/wiki/Xanthium_strumarium Cocklebur (Xanthium strumarium)]
  
 
== Historical importance ==
 
== Historical importance ==
  
  
= Summary of results =
+
== Summary of results ==
  
  
  
= Analytical instrumentation and procedures =
+
== Analytical instrumentation and procedures ==
  
 +
HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.
  
= Chromatograms =
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== Chromatograms ==
  
  
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Sample information
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== Sample information ==
  
 
[[File:Xanthium info.PNG|center|frame|sample information, By R. A. Laursen, Boston University ]]
 
[[File:Xanthium info.PNG|center|frame|sample information, By R. A. Laursen, Boston University ]]
  
= Identified compounds =
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== Identified compounds ==
  
 
[[[SliderGallery rightalign|~HPLC-DAD|.JPG~ UV-Vis|.jpg~ UV-Vis]]]
 
[[[SliderGallery rightalign|~HPLC-DAD|.JPG~ UV-Vis|.jpg~ UV-Vis]]]
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[[Category:Dye Analysis]]
 
[[Category:Dye Analysis]]
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[[Category:Reference Materials]]
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[[Category:Natural Dyes]]

Latest revision as of 11:59, 30 October 2017

[[File:|thumb|Yellow Botanic Gardens]]

Description

Cocklebur (Xanthium strumarium)

Historical importance

Summary of results

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.

Chromatograms

Absorbance at 350nm (mAU)


Sample information

sample information, By R. A. Laursen, Boston University

Identified compounds


Compound RT (min.) MW UV/vis Other
xx xx0 xx xx Comments here
x x x x
x x x x
x x x x

References

[1] [2] [3]