Difference between revisions of "Size exclusion chromatography"
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SEC; GPC; gel permeation chromatography; gel filtration; | SEC; GPC; gel permeation chromatography; gel filtration; | ||
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* Theodore J. Reinhart, 'Glossary of Terms', ''Engineered Plastics'', ASM International, 1988 | * Theodore J. Reinhart, 'Glossary of Terms', ''Engineered Plastics'', ASM International, 1988 |
Revision as of 17:32, 1 May 2016
Description
A type of chromatography that separates compounds based on their molecular size. Size Exclusion Chhromatography (SEC)(formerly called Gel Permeation Chromatography, GPC) uses a separation column filled with a porous, semisolid material. Small molecules pass quickly through the column while larger particles are slowed by their siz and diffusion rates. SEC is primarily used for characterizing molecular weight distributions of polymer units. It has also been used to separate enzymes and amino acids.
Synonyms and Related Terms
SEC; GPC; gel permeation chromatography; gel filtration;
Sources Checked for Data in Record
- Theodore J. Reinhart, 'Glossary of Terms', Engineered Plastics, ASM International, 1988
- Encyclopedia Britannica, http://www.britannica.com Comment: "gel chromatography." Encyclopædia Britannica. 10 Nov. 2004 .
- Wikipedia, the free encyclopedia, at http://www.wikipedia.com Comment: http://en.wikipedia.org/wiki/Gel_permeation_chromatography accessed Nov. 10, 2004