Desert Poplar (Populus pruinosa) LC

Desert Polar tree

Description

Desert Poplar, 灰胡杨, (Populus pruinosa)is a medium-sized deciduous tree. The tree grows up to 10(-20) m tall. The bark has grayish yellow color. It naturally grows in Central and Southwestern Asia, including Western China, [1]

Summary of results

Multiple flavonoids, luteolin, apigenin, chrysoeriol and their glycosides were identified from desert poplar dyed wool samples.

Analytical instrumentation and procedures

The dye was extracted from a thread (0.2-1mg) of the dyed fiber in a solution of pyridine/water/1.0M oxalic acid as described by Mouri and Laursen [2]. The solution was evaporated to dryness under a nitrogen flow, and redissolved in 50 μL MeOH/H2O (1/1); subsequently, 20 μL of dye solution was injected onto HPLC column.

An extract was analyzed on an HPLC-PDA-MS system consisting of a Shimadzu LC-20A high performance liquid chromatography, a Shimadzu SPD-M20A photodiode array detector and a Thermo LTQ XL ion trap mass spectrometer. The separation was performed on a Shim-pack XR-ODS column (3.0 mm × 75 mm, 2.2-μm particle size) and a Phenomenex Luna C18 column (2.0 mm × 150 mm, 3-μm particle size). Columns were eluted with acetonitrile-water gradients containing 0.1% formic acid at a flow rate of 0.3 mL/min.

Chromatograms

Extract of P. Pruinosa dyed textile, Absorbance at 350nm (mAU) by Jian Liu, China National Silk Museum

Compounds identified

Compounds identified, By Jian Liu, China National Silk Museum

Identified compounds

Luteolin UV-Vis

Apigenin UV-Vis

Compound	RT (min.)	MW	UV/vis	Other
Luteolin	35.7	286	348
Apigenin	39.2	270	338

References

[1] Flora of China, efloras.org Populus pruinosa http://www.efloras.org/florataxon.aspx?flora_id=2&taxon_id=200005695 [2] [3]