Berberis dye (berberis thunbergii) LC

Description

Berberis thunbergii, is a dense, deciduous, spiny shrub which grows 0.6 to 2.5 m (2 to 8 ft) high. The plant is also called the Japanese barberry, Thunberg's barberry, or red barberry. It is a species of Berberis, native to Japan and eastern Asia, widely naturalized in China and in North America.

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.

Chromatograms

Absorbance at 350nm (mAU) and compounds identified

Identified compounds

Compound	RT (min.)	MW	UV/vis	Other
Jatrorrhizine	37.4	337	344,428	Comments here
Berberine	43.2	335	348,426

References

[1] [2] [3]