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Excitation Emission Matrix (EEM) A contour plot of excitation wavelength vs emission wavelength vs. fluorescence intensity,

...ch wavelength excites the most molecules to fluoresce. This is called the Excitation maxima. (safflower, madder, sappanwood) Exposing the compound to this maximum excitation wavelength we can measure the emission curve And the point with the highest emission

...dark red being the most intense. The angled bar on the plot is where the excitation and emission wavelengths are identical, thus the detector is saturated at t

Raman spectrum of dry pigment collected at excitation wavelength of 632.8 nm. Image credit: University of Catania, at [http://www.ct.infn.it