Difference between revisions of "Excitation emission matrix (EEM)"

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[[File:Excitation Emission Matrix Horiba.jpg|thumb|Exictation Emission Matrix plot from Horiba: [https://www.horiba.com/int/scientific/technologies/fluorescence-spectroscopy/what-is-an-excitation-emission-matrix-eem/ EEM]]]
 
[[File:Excitation Emission Matrix Horiba.jpg|thumb|Exictation Emission Matrix plot from Horiba: [https://www.horiba.com/int/scientific/technologies/fluorescence-spectroscopy/what-is-an-excitation-emission-matrix-eem/ EEM]]]
 
== Description ==
 
== Description ==
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The Excitation Emission Matrix (EEM) is a specific measurement that is becoming more widely used within the field of fluorescence spectroscopy. An EEM compilation is essentially a three dimensional scan that results in a contour plot of excitation wavelength vs. emission wavelength vs. fluorescence intensity.
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The Excitation Emission Matrix (EEM) is a specific measurement that is becoming more widely used within the field of fluorescence spectroscopy. In a typical fluorescence (emission) measurement, the excitation wavelength is fixed and the detection wavelength varies, while in a fluorescence excitation measurement the detection wavelength is fixed and the excitation wavelength is varied across a region of interest. An emission map is measured by recording the emission spectra resulting from a range of excitation wavelengths and combining them all together. This compilation is a three dimensional scan that results can best be represented as a contour plot of excitation wavelength vs. emission wavelength vs. fluorescence intensity.
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[[File: Madder color.PNG|thumb|EEM color contour map for madder on paper (MFA)
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A type of electromagnetic spectroscopy that analyzes fluorescence from a sample. Fluorescence spectroscopy involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light. A complementary technique is [[absorption spectroscopy]].
 
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In a typical fluorescence (emission) measurement, the excitation wavelength is fixed and the detection wavelength varies, while in a fluorescence excitation measurement the detection wavelength is fixed and the excitation wavelength is varied across a region of interest. An emission map is measured by recording the emission spectra resulting from a range of excitation wavelengths and combining them all together. This is a three dimensional surface data set: emission intensity as a function of excitation and emission wavelengths, and is typically depicted as a contour map.
 
  
 
== Synonyms and Related Terms ==
 
== Synonyms and Related Terms ==

Revision as of 14:48, 21 June 2023

Exictation Emission Matrix plot from Horiba: EEM

Description

The Excitation Emission Matrix (EEM) is a specific measurement that is becoming more widely used within the field of fluorescence spectroscopy. In a typical fluorescence (emission) measurement, the excitation wavelength is fixed and the detection wavelength varies, while in a fluorescence excitation measurement the detection wavelength is fixed and the excitation wavelength is varied across a region of interest. An emission map is measured by recording the emission spectra resulting from a range of excitation wavelengths and combining them all together. This compilation is a three dimensional scan that results can best be represented as a contour plot of excitation wavelength vs. emission wavelength vs. fluorescence intensity. [[File: Madder color.PNG|thumb|EEM color contour map for madder on paper (MFA)

Synonyms and Related Terms

EEM; Excitation-emission matrix spectroscopy; Flourescence spectroscopy; fluorimetry; spectrofluorometry

Resources and Citations

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