Excitation emission matrix (EEM)

Exictation Emission Matrix plot from Horiba: EEM

Description

The Excitation Emission Matrix (EEM) is a specific measurement that is becoming more widely used within the field of fluorescence spectroscopy. In a typical fluorescence (emission) measurement, the excitation wavelength is fixed and the detection wavelength varies, while in a fluorescence excitation measurement the detection wavelength is fixed and the excitation wavelength is varied across a region of interest. An emission map is measured by recording the emission spectra resulting from a range of excitation wavelengths and combining them all together. This compilation is a three dimensional scan that results can best be represented as a contour plot of excitation wavelength vs. emission wavelength vs. fluorescence intensity.

EEM color contour map for madder on paper (MFA)

Synonyms and Related Terms

EEM; Excitation-emission matrix spectroscopy; Fluorescence spectroscopy; Fluorimetry; Spectrofluorimetry

Resources and Citations

• Wikipedia: Fluorescence spectroscopy
• Horiba: EEM