Sawwort (Serratula coronata) LC

[[File:|thumb|Yellow Botanic Gardens]]

Description

Serratula coronata is a perennial growing to 1.5 m (5ft). It prefers moist soil and cannot grow in the shade. It is native to the Eastern Asia and Russia.

Summary of results

Multiple flavonids, including quercetin, luteolin, and their derivatives, were found on the wool samples from Uzbekistan.

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.

Chromatograms

Absorbance at 350nm (mAU)

Sample information

sample information, By R. A. Laursen, Boston University

Identified compounds

Luteolin UV-Vis

3-O-Methylquercetin UV-Vis

Quercetin UV-Vis

Apigenin UV-Vis

Compound	RT (min.)	MW	UV/vis	Other
Quercetin	34.5	302	372	Comments here
Luteolin	35.7	286	348
3-O-Methylquercetin	36.6	316	358
Kaempferol	38.8	286	366
Apigenin	39.2	270	338

References

[1] [2] [3]