Sawwort (Serratula coronata) LC

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[[File:|thumb|Yellow Botanic Gardens]]


Serratula coronata is a perennial growing to 1.5 m (5ft). It prefers moist soil and cannot grow in the shade. It is native to the Eastern Asia and Russia.

Historical importance

Summary of results

Multiple flavonids, including quercetin, luteolin, and their derivatives, were found on the wool samples from Uzbekistan.

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.


Absorbance at 350nm (mAU)

Sample information

sample information, By R. A. Laursen, Boston University

Identified compounds

Luteolin UV-Vis


3-O-Methylquercetin UV-Vis


Quercetin UV-Vis


Apigenin UV-Vis


Compound RT (min.) MW UV/vis Other
Quercetin 34.5 302 372 Comments here
Luteolin 35.7 286 348
3-O-Methylquercetin 36.6 316 358
Kaempferol 38.8 286 366
Apigenin 39.2 270 338


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