Difference between revisions of "Yellowtops (Flaveria haumanii) LC"
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| − | = Summary of results = | + | == Summary of results == |
| − | = Analytical instrumentation and procedures = | + | == Analytical instrumentation and procedures == |
| + | HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode. | ||
| − | = Chromatograms = | + | == Chromatograms == |
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| − | Sample Information | + | == Sample Information == |
[[File:Sample yellowtop.PNG|center|frame|sample information by R. A. Laursen, Boston Univesity]] | [[File:Sample yellowtop.PNG|center|frame|sample information by R. A. Laursen, Boston Univesity]] | ||
| − | = Identified compounds = | + | == Identified compounds == |
| − | [[[SliderGallery rightalign| | + | [[[SliderGallery rightalign|spur.JPG~HPLC-DAD|Quercetin sulfate.JPG~quercetin 3-O-sulfate UV-Vis|Iso sulfate.JPG~Isorhamnetin 3-O-sulfate UV-Vis]]] |
{| class="wikitable" | {| class="wikitable" | ||
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|- | |- | ||
| quercetin 3-O-sulfate | | quercetin 3-O-sulfate | ||
| − | | | + | | 24.4 |
| − | | | + | | 382 |
| 278,348 | | 278,348 | ||
| Comments here | | Comments here | ||
|- | |- | ||
| − | | | + | | isorhamnetin 3-O-sulfate |
| − | | | + | | 27.1 |
| − | | | + | | 394 |
| − | | 280, | + | | 280,350 |
| | | | ||
|- | |- | ||
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| − | [[Category:Analysis | + | [[Category:Dye Analysis]] |
| + | [[Category:Reference Materials]] | ||
| + | [[Category:Natural Dyes]] | ||
Latest revision as of 09:23, 29 September 2017
Description
Historical importance
Summary of results
Analytical instrumentation and procedures
HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.
Chromatograms
Sample Information
Identified compounds
| Compound | RT (min.) | MW | UV/vis | Other |
|---|---|---|---|---|
| quercetin 3-O-sulfate | 24.4 | 382 | 278,348 | Comments here |
| isorhamnetin 3-O-sulfate | 27.1 | 394 | 280,350 | |
| kaempferol | 39.0 | 286 | 280,395,368 | |
| isorhamnetin | 39.0 | 316 | 280,395,368 |
References
[1] [2] [3]