Yellowtops (Flaveria haumanii) LC

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/ picture from Denver Botanic Gardens


Historical importance

Summary of results

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.


Absorbance at 350nm (mAU)

Sample Information

sample information by R. A. Laursen, Boston Univesity

Identified compounds

quercetin 3-O-sulfate UV-Vis

Quercetin sulfate.JPG

Isorhamnetin 3-O-sulfate UV-Vis

Iso sulfate.JPG

Compound RT (min.) MW UV/vis Other
quercetin 3-O-sulfate 24.4 382 278,348 Comments here
isorhamnetin 3-O-sulfate 27.1 394 280,350
kaempferol 39.0 286 280,395,368
isorhamnetin 39.0 316 280,395,368


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