Difference between revisions of "Tetramethyl rhodamine isothiocyanate"
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== Description == | == Description == | ||
| − | A brown color [ | + | A brown color [[fluorescent%20dye|fluorescent dye]] used to detect [[protein|proteins]]. Tetramethyl rhodamine isothiocyanate (TRITC) will react with free hydroxyl or amino groups and thus must be prepared in a water-free solvent. |
== Synonyms and Related Terms == | == Synonyms and Related Terms == | ||
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[[[SliderGallery rightalign|tetramethyl rhodamine isothiocyanate.jpg~Chemical structure]]] | [[[SliderGallery rightalign|tetramethyl rhodamine isothiocyanate.jpg~Chemical structure]]] | ||
| − | == | + | == Physical and Chemical Properties == |
| − | Soluble in water, ethanol, acetone | + | * Soluble in water, ethanol, acetone |
| − | + | * Maximum absorption wavelength = 550 nm | |
| − | Maximum absorption wavelength = 550 nm | + | * Maximum emission wavelength = 570 nm. |
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| − | Maximum emission wavelength = 570 nm. | ||
{| class="wikitable" | {| class="wikitable" | ||
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| − | == | + | == Risks == |
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| − | + | * Moisture sensitive. | |
| + | * Skin irritant. | ||
| + | * Fisher Scientific: [https://fscimage.fishersci.com/msds/19710.htm MSDS] | ||
| − | == | + | ==Resources and Citations== |
* Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, ''Notes for Workshop on New Methods in the Cleaning of Paintings'', J.Paul Getty Trust, Los Angeles, 1990 Comment: mean excitation wavelength of 540 nm (green) and mean emission wavelength=570 nm | * Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, ''Notes for Workshop on New Methods in the Cleaning of Paintings'', J.Paul Getty Trust, Los Angeles, 1990 Comment: mean excitation wavelength of 540 nm (green) and mean emission wavelength=570 nm | ||
| − | * Website | + | * Website: www.mdyn.com/application_notes/technotes - gives max abs. = 554 and max emission = 573 |
| − | * Website | + | * Website: pingu.salk.edu/fcm/fluo.html - gives excitation = 547 and emission = 572 |
* Aldrich Chemical Catalog Comment: p. 1402; abs max=539 | * Aldrich Chemical Catalog Comment: p. 1402; abs max=539 | ||
Latest revision as of 12:03, 8 June 2022
Description
A brown color Fluorescent dye used to detect proteins. Tetramethyl rhodamine isothiocyanate (TRITC) will react with free hydroxyl or amino groups and thus must be prepared in a water-free solvent.
Synonyms and Related Terms
TRITC; Texas red isothiocyanate (not the same as Texas Red®)
Physical and Chemical Properties
- Soluble in water, ethanol, acetone
- Maximum absorption wavelength = 550 nm
- Maximum emission wavelength = 570 nm.
| Composition | C25H21N3O3S |
|---|---|
| CAS | 80724-20-5 |
| Molecular Weight | mol. wt. = 443.52 |
Risks
- Moisture sensitive.
- Skin irritant.
- Fisher Scientific: MSDS
Resources and Citations
- Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990 Comment: mean excitation wavelength of 540 nm (green) and mean emission wavelength=570 nm
- Website: www.mdyn.com/application_notes/technotes - gives max abs. = 554 and max emission = 573
- Website: pingu.salk.edu/fcm/fluo.html - gives excitation = 547 and emission = 572
- Aldrich Chemical Catalog Comment: p. 1402; abs max=539