Tetramethyl rhodamine isothiocyanate

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A brown color Fluorescent dye used to detect proteins. Tetramethyl rhodamine isothiocyanate (TRITC) will react with free hydroxyl or amino groups and thus must be prepared in a water-free solvent.

Synonyms and Related Terms

TRITC; Texas red isothiocyanate (not the same as Texas Red®)

Chemical structure

Tetramethyl rhodamine isothiocyanate.jpg

Physical and Chemical Properties

  • Soluble in water, ethanol, acetone
  • Maximum absorption wavelength = 550 nm
  • Maximum emission wavelength = 570 nm.
Composition C25H21N3O3S
CAS 80724-20-5
Molecular Weight mol. wt. = 443.52


  • Moisture sensitive.
  • Skin irritant.
  • Fisher Scientific: MSDS

Resources and Citations

  • Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990 Comment: mean excitation wavelength of 540 nm (green) and mean emission wavelength=570 nm
  • Website: www.mdyn.com/application_notes/technotes - gives max abs. = 554 and max emission = 573
  • Website: pingu.salk.edu/fcm/fluo.html - gives excitation = 547 and emission = 572
  • Aldrich Chemical Catalog Comment: p. 1402; abs max=539

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