Difference between revisions of "Sawwort (Serratula tinctoria) LC"
| (One intermediate revision by the same user not shown) | |||
| Line 7: | Line 7: | ||
== Historical importance == | == Historical importance == | ||
| + | Saw-wort is a well-known dye plant in Europe and the flowers give a lemon-yellow color. | ||
== Summary of results == | == Summary of results == | ||
| Line 14: | Line 15: | ||
== Analytical instrumentation and procedures == | == Analytical instrumentation and procedures == | ||
| + | HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode. | ||
== Chromatograms == | == Chromatograms == | ||
Latest revision as of 09:22, 29 September 2017
Description
Serratula tinctoria is commonly known as dyer's plumeless saw-wort or saw-wort. It is a native of Europe and a thistle like flower head. It grows in moist soil, full sun to part shade, and grows up to one meter tall.
Historical importance
Saw-wort is a well-known dye plant in Europe and the flowers give a lemon-yellow color.
Summary of results
Flavonoids were identified from the whole plant (aerial part) dyed wool from Turkey.
Analytical instrumentation and procedures
HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.
Chromatograms
Sample information
Identified compounds
| Compound | RT (min.) | MW | UV/vis | Other |
|---|---|---|---|---|
| Luteolin | 36.4 | 286 | 348 | Comments here |
| 3-O-Methylquercetin | 37.3 | 316 | 354 | |
| Apigenin | 40.0 | 270 | 338 |
References
[1] [2] [3]