Sawwort (Serratula tinctoria) LC

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Serratula tinctoria flower Photo by Hectonichus - Own work, CC BY-SA 3.0,


Serratula tinctoria is commonly known as dyer's plumeless saw-wort or saw-wort. It is a native of Europe and a thistle like flower head. It grows in moist soil, full sun to part shade, and grows up to one meter tall.

Historical importance

Saw-wort is a well-known dye plant in Europe and the flowers give a lemon-yellow color.

Summary of results

Flavonoids were identified from the whole plant (aerial part) dyed wool from Turkey.

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.


Absorbance at 350nm (mAU)

Sample information

sample information, By R. A. Laursen, Boston University

Identified compounds

UV-Vis 3-O-Methylquercetin


Apigenin UV-Vis


Luteolin UV-Vis


Compound RT (min.) MW UV/vis Other
Luteolin 36.4 286 348 Comments here
3-O-Methylquercetin 37.3 316 354
Apigenin 40.0 270 338


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