Difference between revisions of "Tetramethyl rhodamine isothiocyanate"
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== Description == | == Description == | ||
| − | A brown color [ | + | A brown color [[fluorescent%20dye|fluorescent dye]] used to detect [[protein|proteins]]. Tetramethyl rhodamine isothiocyanate (TRITC) will react with free hydroxyl or amino groups and thus must be prepared in a water-free solvent. |
== Synonyms and Related Terms == | == Synonyms and Related Terms == | ||
Revision as of 11:48, 10 May 2016
Description
A brown color Fluorescent dye used to detect proteins. Tetramethyl rhodamine isothiocyanate (TRITC) will react with free hydroxyl or amino groups and thus must be prepared in a water-free solvent.
Synonyms and Related Terms
TRITC; Texas red isothiocyanate (not the same as Texas Red®)
Other Properties
Soluble in water, ethanol, acetone
Maximum absorption wavelength = 550 nm
Maximum emission wavelength = 570 nm.
| Composition | C25H21N3O3S |
|---|---|
| CAS | 80724-20-5 |
| Molecular Weight | mol. wt. = 443.52 |
Hazards and Safety
Moisture sensitive. Skin irritant.
Fisher Scientific: MSDS
Additional Information
R. Wolbers, N. Sterman and C. Stavroudis, "Notes for Workshop on New Methods in the Cleaning of Paintings", 1990, GCI, Los Angeles.
Sources Checked for Data in Record
- Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990 Comment: mean excitation wavelength of 540 nm (green) and mean emission wavelength=570 nm
- Website address 1 Comment: www.mdyn.com/application_notes/technotes - gives max abs. = 554 and max emission = 573
- Website address 2 Comment: pingu.salk.edu/fcm/fluo.html - gives excitation = 547 and emission = 572
- Aldrich Chemical Catalog Comment: p. 1402; abs max=539