Tetramethyl rhodamine isothiocyanate

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Description

A brown color fluorescent dye used to detect proteins. Tetramethyl rhodamine isothiocyanate (TRITC) will react with free hydroxyl or amino groups and thus must be prepared in a water-free solvent.

Synonyms and Related Terms

TRITC; Texas red isothiocyanate (not the same as Texas Red®)

Chemical structure

Tetramethyl rhodamine isothiocyanate.jpg


Other Properties

Soluble in water, ethanol, acetone

Maximum absorption wavelength = 550 nm

Maximum emission wavelength = 570 nm.

Composition C25H21N3O3S
CAS 80724-20-5
Molecular Weight mol. wt. = 443.52

Hazards and Safety

Moisture sensitive. Skin irritant.

Fisher Scientific: MSDS

Additional Information

R. Wolbers, N. Sterman and C. Stavroudis, "Notes for Workshop on New Methods in the Cleaning of Paintings", 1990, GCI, Los Angeles.

Sources Checked for Data in Record

  • Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990 Comment: mean excitation wavelength of 540 nm (green) and mean emission wavelength=570 nm
  • Website address 1 Comment: www.mdyn.com/application_notes/technotes - gives max abs. = 554 and max emission = 573
  • Website address 2 Comment: pingu.salk.edu/fcm/fluo.html - gives excitation = 547 and emission = 572
  • Aldrich Chemical Catalog Comment: p. 1402; abs max=539

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