Tetramethyl rhodamine isothiocyanate
Revision as of 13:12, 27 April 2013 by (username removed)
Description
A brown color dye fluorescent dye used to detect proteins. Tetramethyl rhodamine isothiocyanate (TRITC) will react with free hydroxyl or amino groups and thus must be prepared in a water-free solvent.
Synonyms and Related Terms
TRITC; Texas red isothiocyanate (not the same as Texas Red)
Other Properties
Soluble in water, ethanol, acetone
Maximum absorption wavelength = 550 nm
Maximum emission wavelength = 570 nm.
| Composition | C25H21N3O3S |
|---|---|
| CAS | 80724-20-5 |
| Molecular Weight | mol. wt. = 443.52 |
Hazards and Safety
Moisture sensitive. Skin irritant.
Fisher Scientific: MSDS
Additional Information
R. Wolbers, N. Sterman and C. Stavroudis, "Notes for Workshop on New Methods in the Cleaning of Paintings", 1990, GCI, Los Angeles.
Authority
- Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990 Comment: mean excitation wavelength of 540 nm (green) and mean emission wavelength=570 nm
- Website address 1, Website address 1 Comment: www.mdyn.com/application_notes/technotes - gives max abs. = 554 and max emission = 573
- Website address 2 Comment: pingu.salk.edu/fcm/fluo.html - gives excitation = 547 and emission = 572
- Aldrich Chemical Catalog Comment: p. 1402; abs max=539