Brilliant Blue R-250

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Description

A bright blue biological stain. Brilliant Blue R-250 is a dye diazo dye that has been used for staining proteins in paint cross sections and gel electrophoresis. It has also been used as a textile dye and wood stain.

Synonyms and Related Terms

Acid Blue 83; CI 42660; Brillantblau (Deut.); Brilliant Blue R250; Brilliant Blue R; Coomassie R-250;

Chemical structure

Brilliant blue r-250.jpg


Other Properties

Soluble in water, ethanol Insoluble in vegetable oils.

Maximum absorption wavelength = 585 nm.

Composition C45H45N3O7S.Na
CAS 6104-59-2
Molecular Weight mol. wt. = 825.99

Hazards and Safety

Contact may cause irritation.

Mallinckrodt Baker: MSDS

Additional Information

R.Wolbers, G.Landry, "The Use of Direct Reactive Fluorescent Dyes for the Characterization of Binding Media in Cross Sectional Examinations" in Preprints of the AIC Meeting in Vancouver, 1987. M.Johnson, E.Packard, "Methods Used for Identification of Binding Media in Italian paintings of the 15th and 16th Centuries" Studies in Conservation 16:145-164, 1971.

Authority

  • Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990
  • Aldrich Chemical Catalog
  • Sigma Dyes, Stains and Natural Pigments, Infrared Library, Nicolet, 1991-1995

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