Brilliant Blue R-250

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A bright blue biological stain. Brilliant Blue R-250 is a Diazo dye that has been used for staining proteins in paint cross sections and gel Electrophoresis. It has also been used as a textile dye and wood stain.

Synonyms and Related Terms

Acid Blue 83; CI 42660; Brillantblau (Deut.); Brilliant Blue R250; Brilliant Blue R; Coomassie R-250;

Chemical structure

Brilliant blue r-250.jpg


  • Contact may cause irritation.
  • ThermoFiosher: SDS

Physical and Chemical Properties

  • Soluble in water, ethanol Insoluble in vegetable oils.
  • Maximum absorption wavelength = 585 nm.
Composition C45H45N3O7S.Na
CAS 6104-59-2
Molecular Weight mol. wt. = 825.99

Resources and Citations

  • R.Wolbers, G.Landry, "The Use of Direct Reactive Fluorescent Dyes for the Characterization of Binding Media in Cross Sectional Examinations" in Preprints of the AIC Meeting in Vancouver, 1987.
  • M.Johnson, E.Packard, "Methods Used for Identification of Binding Media in Italian paintings of the 15th and 16th Centuries" Studies in Conservation 16:145-164, 1971.
  • Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990
  • Aldrich Chemical Catalog
  • Sigma Dyes, Stains and Natural Pigments, Infrared Library, Nicolet, 1991-1995