Tetramethyl rhodamine isothiocyanate
Revision as of 06:34, 24 July 2013 by (username removed)
Description
A brown color fluorescent dye used to detect proteins. Tetramethyl rhodamine isothiocyanate (TRITC) will react with free hydroxyl or amino groups and thus must be prepared in a water-free solvent.
Synonyms and Related Terms
TRITC; Texas red isothiocyanate (not the same as Texas Red®)
Other Properties
Soluble in water, ethanol, acetone
Maximum absorption wavelength = 550 nm
Maximum emission wavelength = 570 nm.
| Composition | C25H21N3O3S |
|---|---|
| CAS | 80724-20-5 |
| Molecular Weight | mol. wt. = 443.52 |
Hazards and Safety
Moisture sensitive. Skin irritant.
Fisher Scientific: MSDS
Additional Information
R. Wolbers, N. Sterman and C. Stavroudis, "Notes for Workshop on New Methods in the Cleaning of Paintings", 1990, GCI, Los Angeles.
Authority
- Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990 Comment: mean excitation wavelength of 540 nm (green) and mean emission wavelength=570 nm
- Website address 1 Comment: www.mdyn.com/application_notes/technotes - gives max abs. = 554 and max emission = 573
- Website address 2 Comment: pingu.salk.edu/fcm/fluo.html - gives excitation = 547 and emission = 572
- Aldrich Chemical Catalog Comment: p. 1402; abs max=539