Amido black

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Bottle of amido black

Description

A black disazo dye first discovered in 1891 by M. Hoffmann. Amido black is primarily used as a nonfluorescent stain to detect protein. It binds with amino acids to produce a blue-black color but does not stain oil paints or acrylic resins. Amido black reacts with iron to give a weaker, greener color and this variation in color is used in to detect blood in fingerprints.

Synonyms and Related Terms

CI 20470; Acid Black 1; D&C black 1; Amido Black 10B; Amidoschwarz; Naphthol blue black; Aniline blue-black

=Risks

  • May form toxic gases
  • Highly toxic by inhalation and ingestion

Physical and Chemical Properties

  • Soluble in methanol and water.
  • Composition = C22H14N6O9S2Na2
  • CAS = 1064-48-8

Resources and Citations

  • Wikipedia: Amido Black 10B
  • E.Martin "Some Improvements in Technique of Analysis of Paint Media" Studies in Conservation 22:63-67, 1977.
  • Website: http://www.coloria.net/varita.htm - chemical formula
  • Colour Index International online at www.colour-index.org Comment: discoverer - CI#