Amido black

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Bottle of amido black


A black disazo dye first discovered in 1891 by M. Hoffmann. Amido black is primarily used as a nonfluorescent stain to detect Protein. It binds with proteins to produce a blue-black color but does not stain oil paints or acrylic resins.

Synonyms and Related Terms

AB2; CI 20470; Acid Black 1 ; D&C black 1; Amido Black 10B; Amidoschwarz; Naphthol blue black

Physical and Chemical Properties

Soluble in methanol and water.

Composition C22H14N6O9S2Na2
CAS 1064-48-8

Resources and Citations

  • E.Martin "Some Improvements in Technique of Analysis of Paint Media" Studies in Conservation 22:63-67, 1977.
  • Colour Index International online at Comment: discoverer - CI#