Berberis dye (berberis Julianae) LC

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Berberis julianae bush, photo by X. Zhang at the Arnold Arboretum, Boston


Berberis julianae, also called Mahonia julianae, is a flowering evergreen shrub native to Central China. It is widely grown as an ornamental in other temperate regions. It grows to 6' to 8' tall and 4' to 6' wide.

Historical importance

Summary of results

Analytical instrumentation and procedures

HPLC-DAD-MS analysis was performed with an Agilent 1100 liquid chromatography system consisting of an automatic injector, a gradient pump, a HP series 1100 DAD, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. Separations were done on a Vydac 214TP52 analytical column (2.1 mm diameterX250 mm; 5-ím particle size). The column was eluted at a flow rate of 0.2 mL/min with a tertiary gradient of water (A),acetonitrile (B), and 1% (v/v) aqueous formic acid (C) with the following elution program: 0 min, 90% A, 5% B, 5% C; 0-55 min, a linear gradient to 35% A, 60% B, 5% C; 55-60 min, a linear gradient elution to 15% A, 80% B, 5% C; 60-62 min, isocratic elution at 15% A, 80% B, 5% C; 62-70 min gradient elution to 90% A, 5% B, 5% C; and reequilibration with the latter solvent for 15 min. The mass spectrometer was run both in the negative and positive ion mode.


Absorbance at 350nm (mAU), By X. Zhang

sample information

Identified compounds

Compound RT (min.) MW UV/vis Other
Jatrorrhizine 29.4 337 344,426
Berberine 32.7 335 344,426 co-elutes with palmatine
Palmatine 32.7 351 344,426 co-elutes with berberin


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