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A non-fluorescent chemical that reacts with amino acids, peptides, and proteins to form a highly fluorescent compound. Fluorescamine is prepared as a 0.05% water-free solution in acetone. Once reacted with a primary amine compound, fluorescamine will fluoresce a bright blue-white under ultraviolet light. The fluorescence may be hard to distinguish from the natural autofluorescence of many proteins. For maximum shelf life, fluorescamine should be stored in dry, dark conditions.

Synonyms and Related Terms

4-phenylspiro[furan-2(3H),1'(3'H)-isobenzofuran]-3,3'-dione; Flur; fluram; Ro-20-7234


  • Sensitive to light and moisture.
  • Fisher Scientific: MSDS

Other Properties

  • Soluble in acetone and other polar solvents.
  • Maximum absorption wavelengths = 235,276, 284, 306 nm.
Composition C17H10O4
CAS 38183-12-9
Melting Point 154-155 C
Molecular Weight mol. wt. = 278.26

Resources and Citations

  • Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990
  • The Merck Index, Martha Windholz (ed.), Merck Research Labs, Rahway NJ, 10th edition, 1983 Comment: entry 4193
  • Sigma Dyes, Stains and Natural Pigments, Infrared Library, Nicolet, 1991-1995 Comment: OMNIC: formula= C17H10O4, CAS= 38183-12-9

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