Difference between revisions of "Fluorochrome"
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A fluorescent substance used to detect the presence of another material. Examples of fluorochromes used in the detection of art materials are: [[berberine sulfate]], [[acridine orange]], [[acridine yellow]], [[Auramine O]], [[Blancophor R]], [[cycloheptaamylose dansyl chloride]], [[dichlorofluorescein]], [[fluorescein isothiocyanate]], [[Lissamine Rhodamine B Sulfonyl Chloride]], [[primuline]], [[pyronine Y]], [[Rhodamine B]], [[rosaniline]], [[serotonin]], [[Tetracycline]], and [[Texas Red]]. | A fluorescent substance used to detect the presence of another material. Examples of fluorochromes used in the detection of art materials are: [[berberine sulfate]], [[acridine orange]], [[acridine yellow]], [[Auramine O]], [[Blancophor R]], [[cycloheptaamylose dansyl chloride]], [[dichlorofluorescein]], [[fluorescein isothiocyanate]], [[Lissamine Rhodamine B Sulfonyl Chloride]], [[primuline]], [[pyronine Y]], [[Rhodamine B]], [[rosaniline]], [[serotonin]], [[Tetracycline]], and [[Texas Red]]. | ||
+ | |||
+ | {| class="wikitable" | ||
+ | |- | ||
+ | ! Fluorochrome | ||
+ | ! Excitation Wavelength | ||
+ | ! Emission Wavelength | ||
+ | |- | ||
+ | | Acid Fuchsin | ||
+ | | 540 | ||
+ | | 630 | ||
+ | |- | ||
+ | | Acridine Orange<br />(Bound to DNA) | ||
+ | | 502 | ||
+ | | 526 | ||
+ | |- | ||
+ | | Acridine Red | ||
+ | | 455-600 | ||
+ | | 560-680 | ||
+ | |- | ||
+ | | Acridine Yellow | ||
+ | | 470 | ||
+ | | 550 | ||
+ | |- | ||
+ | | Acriflavin | ||
+ | | 436 | ||
+ | | 520 | ||
+ | |- | ||
+ | | AFA (Acriflavin<br />Feulgen SITSA) | ||
+ | | 355-425 | ||
+ | | 460 | ||
+ | |- | ||
+ | | Alizarin Complexon | ||
+ | | 530-560 | ||
+ | | 580 | ||
+ | |- | ||
+ | | Alizarin Red | ||
+ | | 530-560 | ||
+ | | 580 | ||
+ | |- | ||
+ | | Allophycocyanin | ||
+ | | 650 | ||
+ | | 661 | ||
+ | |- | ||
+ | | ACMA | ||
+ | | 430 | ||
+ | | 474 | ||
+ | |- | ||
+ | | AMCA-S, AMC | ||
+ | | 345 | ||
+ | | 445 | ||
+ | |- | ||
+ | | Aminoactinomycin D | ||
+ | | 555 | ||
+ | | 655 | ||
+ | |- | ||
+ | | 7-Aminoactinomycin D-AAD | ||
+ | | 546 | ||
+ | | 647 | ||
+ | |- | ||
+ | | Aminocoumarin | ||
+ | | 350 | ||
+ | | 445 | ||
+ | |- | ||
+ | | Anthroyl Stearate | ||
+ | | 361-381 | ||
+ | | 446 | ||
+ | |- | ||
+ | | Astrazon Brilliant Red 4G | ||
+ | | 500 | ||
+ | | 585 | ||
+ | |- | ||
+ | | Astrazon Orange R | ||
+ | | 470 | ||
+ | | 540 | ||
+ | |- | ||
+ | | Astrazon Red 6B | ||
+ | | 520 | ||
+ | | 595 | ||
+ | |- | ||
+ | | Astrazon Yellow 7 GLL | ||
+ | | 450 | ||
+ | | 480 | ||
+ | |- | ||
+ | | Atabrine | ||
+ | | 436 | ||
+ | | 490 | ||
+ | |- | ||
+ | | Auramine | ||
+ | | 460 | ||
+ | | 550 | ||
+ | |- | ||
+ | | Aurophosphine | ||
+ | | 450-490 | ||
+ | | 515 | ||
+ | |- | ||
+ | | Aurophosphine G | ||
+ | | 450 | ||
+ | | 580 | ||
+ | |- | ||
+ | | BAO 9-(Bisamino-phenyloxadiazole) | ||
+ | | 365 | ||
+ | | 395 | ||
+ | |- | ||
+ | | BCECF | ||
+ | | 505 | ||
+ | | 530 | ||
+ | |- | ||
+ | | Berberine Sulphate | ||
+ | | 430 | ||
+ | | 550 | ||
+ | |- | ||
+ | | Bisbenzamide | ||
+ | | 360 | ||
+ | | 600-610 | ||
+ | |- | ||
+ | | BOBO-1, BO-PRO-1 | ||
+ | | 462 | ||
+ | | 481 | ||
+ | |- | ||
+ | | Blancophor FFG Solution | ||
+ | | 390 | ||
+ | | 470 | ||
+ | |- | ||
+ | | Blancophor SV | ||
+ | | 370 | ||
+ | | 435 | ||
+ | |- | ||
+ | | Bodipy Fl | ||
+ | | 503 | ||
+ | | 512 | ||
+ | |- | ||
+ | | Bodipy TMR | ||
+ | | 542 | ||
+ | | 574 | ||
+ | |- | ||
+ | | Bodipy TR | ||
+ | | 589 | ||
+ | | 617 | ||
+ | |- | ||
+ | | BOPRO 1 | ||
+ | | 462 | ||
+ | | 481 | ||
+ | |- | ||
+ | | Brilliant Sulpho-flavin FF | ||
+ | | 430 | ||
+ | | 520 | ||
+ | |- | ||
+ | | Calcein | ||
+ | | 494 | ||
+ | | 517 | ||
+ | |- | ||
+ | | Calcien Blue | ||
+ | | 370 | ||
+ | | 435 | ||
+ | |- | ||
+ | | Calcium Green | ||
+ | | 505 | ||
+ | | 532 | ||
+ | |- | ||
+ | | Calcium Orange | ||
+ | | 549 | ||
+ | | 576 | ||
+ | |- | ||
+ | | Calcofluor RW Solution | ||
+ | | 370 | ||
+ | | 440 | ||
+ | |- | ||
+ | | Calcofluor White | ||
+ | | 440 | ||
+ | | 500-520 | ||
+ | |- | ||
+ | | Calcofluor White ABT Solution | ||
+ | | 380 | ||
+ | | 475 | ||
+ | |- | ||
+ | | Calcofluor White Standard Solution | ||
+ | | 365 | ||
+ | | 435 | ||
+ | |- | ||
+ | | 5-(and 6-)carboxy SNARF-1 indicator | ||
+ | | 548(low pH)<br />576(high pH) | ||
+ | | 587(low pH)<br />635(high pH) | ||
+ | |- | ||
+ | | 6-Carboxyrhodamine 6G | ||
+ | | 525 | ||
+ | | 555 | ||
+ | |- | ||
+ | | Cascade Blue | ||
+ | | 400 | ||
+ | | 425 | ||
+ | |- | ||
+ | | Catecholamine | ||
+ | | 410 | ||
+ | | 470 | ||
+ | |- | ||
+ | | Chinacrine | ||
+ | | 450-490 | ||
+ | | 515 | ||
+ | |- | ||
+ | | CL-NERF | ||
+ | | 504(low pH)<br />514(high pH) | ||
+ | | 587(low pH)<br />540(high pH) | ||
+ | |- | ||
+ | | Coriphosphine O | ||
+ | | 460 | ||
+ | | 575 | ||
+ | |- | ||
+ | | Coumarin-Phalloidin | ||
+ | | 387 | ||
+ | | 470 | ||
+ | |- | ||
+ | | CY3.18 | ||
+ | | 554 | ||
+ | | 568 | ||
+ | |- | ||
+ | | CY5.18 | ||
+ | | 649 | ||
+ | | 666 | ||
+ | |- | ||
+ | | CY7 | ||
+ | | 710 | ||
+ | | 805 | ||
+ | |- | ||
+ | | DANS (1-DimethylAmino-Naphthaline-5-Sulphonic Acid) | ||
+ | | 340 | ||
+ | | 525 | ||
+ | |- | ||
+ | | DANSA (DiaminoNaphthyl-Sulphonic Acid) | ||
+ | | 340-380 | ||
+ | | 430 | ||
+ | |- | ||
+ | | Dansyl NH-CH3 in water | ||
+ | | 340 | ||
+ | | 578 | ||
+ | |- | ||
+ | | DAPI | ||
+ | | 350 | ||
+ | | 470 | ||
+ | |- | ||
+ | | DiA | ||
+ | | 456 | ||
+ | | 590 | ||
+ | |- | ||
+ | | Diamino Phenyl Oxydiazole (DAO) | ||
+ | | 280 | ||
+ | | 460 | ||
+ | |- | ||
+ | | Di-8-ANEPPS | ||
+ | | 488 | ||
+ | | 605 | ||
+ | |- | ||
+ | | Dimethylamino-5-Sulphonic Acid | ||
+ | | 310-370 | ||
+ | | 520 | ||
+ | |- | ||
+ | | DiI [DiIC18(3)] | ||
+ | | 549 | ||
+ | | 565 | ||
+ | |- | ||
+ | | DiO [DiOC18(3)] | ||
+ | | 484 | ||
+ | | 501 | ||
+ | |- | ||
+ | | Diphenyl Brilliant Flavine 7GFF | ||
+ | | 430 | ||
+ | | 520 | ||
+ | |- | ||
+ | | DM-NERF | ||
+ | | 497(low pH)<br />510(high pH) | ||
+ | | 527(low pH)<br />536(high pH) | ||
+ | |- | ||
+ | | Dopamine | ||
+ | | 340 | ||
+ | | 490-520 | ||
+ | |- | ||
+ | | ELF-97 alcohol | ||
+ | | 345 | ||
+ | | 530 | ||
+ | |- | ||
+ | | Eosin | ||
+ | | 525 | ||
+ | | 545 | ||
+ | |- | ||
+ | | Erythrosin ITC | ||
+ | | 530 | ||
+ | | 558 | ||
+ | |- | ||
+ | | Ethidium Bromide | ||
+ | | 510 | ||
+ | | 595 | ||
+ | |- | ||
+ | | Euchrysin | ||
+ | | 430 | ||
+ | | 540 | ||
+ | |- | ||
+ | | FIF (Formaldehyde Induced Fluorescence) | ||
+ | | 405 | ||
+ | | 435 | ||
+ | |- | ||
+ | | Flazo Orange | ||
+ | | 375-530 | ||
+ | | 612 | ||
+ | |- | ||
+ | | Fluorescein | ||
+ | | 494 | ||
+ | | 518 | ||
+ | |- | ||
+ | | Fluorescein Iso-thiocyanate (FITC) | ||
+ | | 490 | ||
+ | | 525 | ||
+ | |- | ||
+ | | Fluo 3 | ||
+ | | 485 | ||
+ | | 503 | ||
+ | |- | ||
+ | | FM1-43 | ||
+ | | 479 | ||
+ | | 598 | ||
+ | |- | ||
+ | | Fura-2 | ||
+ | | 363(low [Ca2+])<br />335(high [Ca2+]) | ||
+ | | 512(low [Ca2+])<br />505(high [Ca2+]) | ||
+ | |- | ||
+ | | Fura Red | ||
+ | | 472(low [Ca2+])<br />436(high [Ca2+]) | ||
+ | | 657(low [Ca2+])<br />637(high [Ca2+]) | ||
+ | |- | ||
+ | | Genacryl Brilliant Red B | ||
+ | | 520 | ||
+ | | 590 | ||
+ | |- | ||
+ | | Genacryl Brilliant Yellow 10GF | ||
+ | | 430 | ||
+ | | 485 | ||
+ | |- | ||
+ | | Genacryl Pink 3G | ||
+ | | 470 | ||
+ | | 583 | ||
+ | |- | ||
+ | | Genacryl Yellow 5GF | ||
+ | | 430 | ||
+ | | 475 | ||
+ | |- | ||
+ | | Gloxalic Acid | ||
+ | | 405 | ||
+ | | 460 | ||
+ | |- | ||
+ | | Granular Blue | ||
+ | | 355 | ||
+ | | 425 | ||
+ | |- | ||
+ | | Haematoporphyrin | ||
+ | | 530-560 | ||
+ | | 580 | ||
+ | |- | ||
+ | | Hoechst 33258, 33342 (Bound to DNA) | ||
+ | | 352 | ||
+ | | 461 | ||
+ | |- | ||
+ | | 3-Hydroxypyrene-5,-8,10-TriSulfonic Acid | ||
+ | | 403 | ||
+ | | 513 | ||
+ | |- | ||
+ | | 7-Hydroxy-4-methylcoumarin | ||
+ | | 360 | ||
+ | | 455 | ||
+ | |- | ||
+ | | 5-Hydroxy-Tryptamine (5-HT) | ||
+ | | 380-415 | ||
+ | | 520-530 | ||
+ | |- | ||
+ | | Indo-1 | ||
+ | | 350 | ||
+ | | 405-482 | ||
+ | |- | ||
+ | | Intrawhite Cf Liquid | ||
+ | | 360 | ||
+ | | 430 | ||
+ | |- | ||
+ | | Leucophor PAF | ||
+ | | 370 | ||
+ | | 430 | ||
+ | |- | ||
+ | | Leucophor SF | ||
+ | | 380 | ||
+ | | 465 | ||
+ | |- | ||
+ | | Leucophor WS | ||
+ | | 395 | ||
+ | | 465 | ||
+ | |- | ||
+ | | Lissamine Rhodamine B200 (RD200) | ||
+ | | 575 | ||
+ | | 595 | ||
+ | |- | ||
+ | | Lucifer Yellow CH | ||
+ | | 425 | ||
+ | | 528 | ||
+ | |- | ||
+ | | Lucifer Yellow VS | ||
+ | | 430 | ||
+ | | 535 | ||
+ | |- | ||
+ | | LysoSensor Blue DND-192, DND-167 | ||
+ | | 374 | ||
+ | | 425 | ||
+ | |- | ||
+ | | LysoSensor Green DND-153, DND-189 | ||
+ | | 442 | ||
+ | | 505 | ||
+ | |- | ||
+ | | LysoSensor Yellow/Blue | ||
+ | | 384(low pH)<br />329(high pH) | ||
+ | | 540(low pH)<br />440(high pH) | ||
+ | |- | ||
+ | | LysoTracker Green | ||
+ | | 504 | ||
+ | | 511 | ||
+ | |- | ||
+ | | LysoTracker Yellow | ||
+ | | 534 | ||
+ | | 551 | ||
+ | |- | ||
+ | | LysoTracker Red | ||
+ | | 577 | ||
+ | | 592 | ||
+ | |- | ||
+ | | Magdala Red | ||
+ | | 524 | ||
+ | | 600 | ||
+ | |- | ||
+ | | Magnesium Green | ||
+ | | 506 | ||
+ | | 531 | ||
+ | |- | ||
+ | | Magnesium Orange | ||
+ | | 550 | ||
+ | | 575 | ||
+ | |- | ||
+ | | Maxilon Brilliant Flavin 10 GFF | ||
+ | | 450 | ||
+ | | 495 | ||
+ | |- | ||
+ | | Maxilon Brilliant Flavin 8 GFF | ||
+ | | 460 | ||
+ | | 495 | ||
+ | |- | ||
+ | | Mitotracker Green FM | ||
+ | | 490 | ||
+ | | 516 | ||
+ | |- | ||
+ | | Mitotracker Orange CMTMRos | ||
+ | | 551 | ||
+ | | 576 | ||
+ | |- | ||
+ | | MPS (Methyl Green Pyronine Stilbene) | ||
+ | | 364 | ||
+ | | 395 | ||
+ | |- | ||
+ | | Mithramycin | ||
+ | | 450 | ||
+ | | 570 | ||
+ | |- | ||
+ | | NBD | ||
+ | | 465 | ||
+ | | 535 | ||
+ | |- | ||
+ | | NBD Amine | ||
+ | | 450 | ||
+ | | 530 | ||
+ | |- | ||
+ | | Nile Red | ||
+ | | 515-530 | ||
+ | | 525-605 | ||
+ | |- | ||
+ | | Nitrobenzoxadidole | ||
+ | | 460-470 | ||
+ | | 510-650 | ||
+ | |- | ||
+ | | Noradrenaline | ||
+ | | 340 | ||
+ | | 490-520 | ||
+ | |- | ||
+ | | Nuclear Fast Red | ||
+ | | 289-530 | ||
+ | | 580 | ||
+ | |- | ||
+ | | Nuclear Yellow | ||
+ | | 365 | ||
+ | | 495 | ||
+ | |- | ||
+ | | Nylosan Brilliant Flavin E8G | ||
+ | | 460 | ||
+ | | 510 | ||
+ | |- | ||
+ | | Oregon Green 488 fluorophore | ||
+ | | 496 | ||
+ | | 524 | ||
+ | |- | ||
+ | | Oregon Green 500 fluorophore | ||
+ | | 503 | ||
+ | | 522 | ||
+ | |- | ||
+ | | Oregon Green 514 fluorophore | ||
+ | | 511 | ||
+ | | 530 | ||
+ | |- | ||
+ | | Pararosaniline (Feulgen) | ||
+ | | 570 | ||
+ | | 625 | ||
+ | |- | ||
+ | | Phorwite AR Solution | ||
+ | | 360 | ||
+ | | 430 | ||
+ | |- | ||
+ | | Phorwite BKL | ||
+ | | 370 | ||
+ | | 430 | ||
+ | |- | ||
+ | | Phorwite Rev | ||
+ | | 380 | ||
+ | | 430 | ||
+ | |- | ||
+ | | Phorwite RPA | ||
+ | | 375 | ||
+ | | 430 | ||
+ | |- | ||
+ | | Phosphine 3R | ||
+ | | 465 | ||
+ | | 565 | ||
+ | |- | ||
+ | | Phosphine R | ||
+ | | 480-565 | ||
+ | | 578 | ||
+ | |- | ||
+ | | Pontochrome Blue Black | ||
+ | | 535-553 | ||
+ | | 605 | ||
+ | |- | ||
+ | | POPO-1, PO-PRO-1 | ||
+ | | 434 | ||
+ | | 456 | ||
+ | |- | ||
+ | | Primuline | ||
+ | | 410 | ||
+ | | 550 | ||
+ | |- | ||
+ | | Procion Yellow | ||
+ | | 470 | ||
+ | | 600 | ||
+ | |- | ||
+ | | Propidium Iodide | ||
+ | | 536 | ||
+ | | 617 | ||
+ | |- | ||
+ | | Pyronine | ||
+ | | 410 | ||
+ | | 540 | ||
+ | |- | ||
+ | | Pyronine B | ||
+ | | 540-590 | ||
+ | | 560-650 | ||
+ | |- | ||
+ | | Pyrozal Brilliant Flavin 7GF | ||
+ | | 365 | ||
+ | | 495 | ||
+ | |- | ||
+ | | Quinacrine Mustard | ||
+ | | 423 | ||
+ | | 503 | ||
+ | |- | ||
+ | | R-phycoerythrin | ||
+ | | 565 | ||
+ | | 575 | ||
+ | |- | ||
+ | | Rhodamine 110 | ||
+ | | 496 | ||
+ | | 520 | ||
+ | |- | ||
+ | | Rhodamine 123 | ||
+ | | 511 | ||
+ | | 534 | ||
+ | |- | ||
+ | | Rhodamine 5 GLD | ||
+ | | 470 | ||
+ | | 565 | ||
+ | |- | ||
+ | | Rhodamine 6G | ||
+ | | 526 | ||
+ | | 555 | ||
+ | |- | ||
+ | | Rhodamine B | ||
+ | | 540 | ||
+ | | 625 | ||
+ | |- | ||
+ | | Rhodamine B 200 | ||
+ | | 523-557 | ||
+ | | 595 | ||
+ | |- | ||
+ | | Rhodamine B Extra | ||
+ | | 550 | ||
+ | | 605 | ||
+ | |- | ||
+ | | Rhodamine BB | ||
+ | | 540 | ||
+ | | 580 | ||
+ | |- | ||
+ | | Rhodamine BG | ||
+ | | 540 | ||
+ | | 572 | ||
+ | |- | ||
+ | | Rhodamine Green fluorophore | ||
+ | | 502 | ||
+ | | 527 | ||
+ | |- | ||
+ | | Rhodamine Red | ||
+ | | 570 | ||
+ | | 590 | ||
+ | |- | ||
+ | | Rhodamine WT | ||
+ | | 530 | ||
+ | | 555 | ||
+ | |- | ||
+ | | Rhodol Green fluorophore | ||
+ | | 499 | ||
+ | | 525 | ||
+ | |- | ||
+ | | Rose Bengal | ||
+ | | 540 | ||
+ | | 550-600 | ||
+ | |- | ||
+ | | Serotonin | ||
+ | | 365 | ||
+ | | 520-540 | ||
+ | |- | ||
+ | | Sevron Brilliant Red 2B | ||
+ | | 520 | ||
+ | | 595 | ||
+ | |- | ||
+ | | Sevron Brilliant Red 4G | ||
+ | | 500 | ||
+ | | 583 | ||
+ | |- | ||
+ | | Sevron Brilliant Red B | ||
+ | | 530 | ||
+ | | 590 | ||
+ | |- | ||
+ | | Sevron Orange | ||
+ | | 440 | ||
+ | | 530 | ||
+ | |- | ||
+ | | Sevron Yellow L | ||
+ | | 430 | ||
+ | | 490 | ||
+ | |- | ||
+ | | SITS (Primuline) | ||
+ | | 395-425 | ||
+ | | 450 | ||
+ | |- | ||
+ | | SITS (Stilbene Isothiosulphonic Acid) | ||
+ | | 365 | ||
+ | | 460 | ||
+ | |- | ||
+ | | Sodium Green | ||
+ | | 507 | ||
+ | | 535 | ||
+ | |- | ||
+ | | Stilbene | ||
+ | | 335 | ||
+ | | 440 | ||
+ | |- | ||
+ | | Snarf 1 | ||
+ | | 563 | ||
+ | | 639 | ||
+ | |- | ||
+ | | Sulpho Rhodamine B Can C | ||
+ | | 520 | ||
+ | | 595 | ||
+ | |- | ||
+ | | Sulpho Rhodamine G Extra | ||
+ | | 470 | ||
+ | | 570 | ||
+ | |- | ||
+ | | SYTOX Green nucleic acid stain | ||
+ | | 504 | ||
+ | | 523 | ||
+ | |- | ||
+ | | SYTO Green fluorescent nucleic acid stains | ||
+ | | 494 ± 6 | ||
+ | | 515 ± 7 | ||
+ | |- | ||
+ | | | ||
+ | | 515 ± 7 | ||
+ | | 543 ± 13 | ||
+ | |- | ||
+ | | SYTO 17 red fluorescent nucleic acid stain | ||
+ | | 621 | ||
+ | | 634 | ||
+ | |- | ||
+ | | Tetracycline | ||
+ | | 390 | ||
+ | | 560 | ||
+ | |- | ||
+ | | TRITC (Tetramethyl Rhodamine Isothiocyanate) | ||
+ | | 557 | ||
+ | | 576 | ||
+ | |- | ||
+ | | Texas Red | ||
+ | | 596 | ||
+ | | 615 | ||
+ | |- | ||
+ | | Thiazine Red R | ||
+ | | 510 | ||
+ | | 580 | ||
+ | |- | ||
+ | | Thioflavin S | ||
+ | | 430 | ||
+ | | 550 | ||
+ | |- | ||
+ | | Thioflavin TCN | ||
+ | | 350 | ||
+ | | 460 | ||
+ | |- | ||
+ | | Thioflavin 5 | ||
+ | | 430 | ||
+ | | 550 | ||
+ | |- | ||
+ | | Thiolyte | ||
+ | | 370-385 | ||
+ | | 477-484 | ||
+ | |- | ||
+ | | Thiozol Orange | ||
+ | | 453 | ||
+ | | 480 | ||
+ | |- | ||
+ | | Tinopol CBS | ||
+ | | 390 | ||
+ | | 430 | ||
+ | |- | ||
+ | | TOTO 1, TO-RRO-1 | ||
+ | | 514 | ||
+ | | 533 | ||
+ | |- | ||
+ | | TOTO 3, TO-PRO-3 | ||
+ | | 642 | ||
+ | | 661 | ||
+ | |- | ||
+ | | True Blue | ||
+ | | 365 | ||
+ | | 420-430 | ||
+ | |- | ||
+ | | Ultralite | ||
+ | | 656 | ||
+ | | 678 | ||
+ | |- | ||
+ | | Uranine B | ||
+ | | 420 | ||
+ | | 520 | ||
+ | |- | ||
+ | | Uvitex SFC | ||
+ | | 365 | ||
+ | | 435 | ||
+ | |- | ||
+ | | X-Rhodamine | ||
+ | | 580 | ||
+ | | 605 | ||
+ | |- | ||
+ | | Xylene Orange | ||
+ | | 546 | ||
+ | | 580 | ||
+ | |- | ||
+ | | XRITC | ||
+ | | 582 | ||
+ | | 601 | ||
+ | |- | ||
+ | | YOYO-1, YOYO-PRO-1 | ||
+ | | 491 | ||
+ | | 509 | ||
+ | |- | ||
+ | | YOYO-3, YOYO-PRO-3 | ||
+ | | 612 | ||
+ | | 631 | ||
+ | |} | ||
== Synonyms and Related Terms == | == Synonyms and Related Terms == | ||
Line 8: | Line 791: | ||
==Resources and Citations== | ==Resources and Citations== | ||
+ | |||
+ | * Olympus LS: [https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/fluorescence/fluorotable2/ Fluorochrome table] | ||
* Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, ''Notes for Workshop on New Methods in the Cleaning of Paintings'', J.Paul Getty Trust, Los Angeles, 1990 | * Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, ''Notes for Workshop on New Methods in the Cleaning of Paintings'', J.Paul Getty Trust, Los Angeles, 1990 |
Latest revision as of 11:37, 16 October 2022
Description
A fluorescent substance used to detect the presence of another material. Examples of fluorochromes used in the detection of art materials are: Berberine sulfate, Acridine orange, Acridine yellow, Auramine O, Blancophor R, Cycloheptaamylose dansyl chloride, Dichlorofluorescein, Fluorescein isothiocyanate, Lissamine Rhodamine B Sulfonyl Chloride, Primuline, Pyronine Y, Rhodamine B, Rosaniline, Serotonin, Tetracycline, and Texas Red.
Fluorochrome | Excitation Wavelength | Emission Wavelength |
---|---|---|
Acid Fuchsin | 540 | 630 |
Acridine Orange (Bound to DNA) |
502 | 526 |
Acridine Red | 455-600 | 560-680 |
Acridine Yellow | 470 | 550 |
Acriflavin | 436 | 520 |
AFA (Acriflavin Feulgen SITSA) |
355-425 | 460 |
Alizarin Complexon | 530-560 | 580 |
Alizarin Red | 530-560 | 580 |
Allophycocyanin | 650 | 661 |
ACMA | 430 | 474 |
AMCA-S, AMC | 345 | 445 |
Aminoactinomycin D | 555 | 655 |
7-Aminoactinomycin D-AAD | 546 | 647 |
Aminocoumarin | 350 | 445 |
Anthroyl Stearate | 361-381 | 446 |
Astrazon Brilliant Red 4G | 500 | 585 |
Astrazon Orange R | 470 | 540 |
Astrazon Red 6B | 520 | 595 |
Astrazon Yellow 7 GLL | 450 | 480 |
Atabrine | 436 | 490 |
Auramine | 460 | 550 |
Aurophosphine | 450-490 | 515 |
Aurophosphine G | 450 | 580 |
BAO 9-(Bisamino-phenyloxadiazole) | 365 | 395 |
BCECF | 505 | 530 |
Berberine Sulphate | 430 | 550 |
Bisbenzamide | 360 | 600-610 |
BOBO-1, BO-PRO-1 | 462 | 481 |
Blancophor FFG Solution | 390 | 470 |
Blancophor SV | 370 | 435 |
Bodipy Fl | 503 | 512 |
Bodipy TMR | 542 | 574 |
Bodipy TR | 589 | 617 |
BOPRO 1 | 462 | 481 |
Brilliant Sulpho-flavin FF | 430 | 520 |
Calcein | 494 | 517 |
Calcien Blue | 370 | 435 |
Calcium Green | 505 | 532 |
Calcium Orange | 549 | 576 |
Calcofluor RW Solution | 370 | 440 |
Calcofluor White | 440 | 500-520 |
Calcofluor White ABT Solution | 380 | 475 |
Calcofluor White Standard Solution | 365 | 435 |
5-(and 6-)carboxy SNARF-1 indicator | 548(low pH) 576(high pH) |
587(low pH) 635(high pH) |
6-Carboxyrhodamine 6G | 525 | 555 |
Cascade Blue | 400 | 425 |
Catecholamine | 410 | 470 |
Chinacrine | 450-490 | 515 |
CL-NERF | 504(low pH) 514(high pH) |
587(low pH) 540(high pH) |
Coriphosphine O | 460 | 575 |
Coumarin-Phalloidin | 387 | 470 |
CY3.18 | 554 | 568 |
CY5.18 | 649 | 666 |
CY7 | 710 | 805 |
DANS (1-DimethylAmino-Naphthaline-5-Sulphonic Acid) | 340 | 525 |
DANSA (DiaminoNaphthyl-Sulphonic Acid) | 340-380 | 430 |
Dansyl NH-CH3 in water | 340 | 578 |
DAPI | 350 | 470 |
DiA | 456 | 590 |
Diamino Phenyl Oxydiazole (DAO) | 280 | 460 |
Di-8-ANEPPS | 488 | 605 |
Dimethylamino-5-Sulphonic Acid | 310-370 | 520 |
DiI [DiIC18(3)] | 549 | 565 |
DiO [DiOC18(3)] | 484 | 501 |
Diphenyl Brilliant Flavine 7GFF | 430 | 520 |
DM-NERF | 497(low pH) 510(high pH) |
527(low pH) 536(high pH) |
Dopamine | 340 | 490-520 |
ELF-97 alcohol | 345 | 530 |
Eosin | 525 | 545 |
Erythrosin ITC | 530 | 558 |
Ethidium Bromide | 510 | 595 |
Euchrysin | 430 | 540 |
FIF (Formaldehyde Induced Fluorescence) | 405 | 435 |
Flazo Orange | 375-530 | 612 |
Fluorescein | 494 | 518 |
Fluorescein Iso-thiocyanate (FITC) | 490 | 525 |
Fluo 3 | 485 | 503 |
FM1-43 | 479 | 598 |
Fura-2 | 363(low [Ca2+]) 335(high [Ca2+]) |
512(low [Ca2+]) 505(high [Ca2+]) |
Fura Red | 472(low [Ca2+]) 436(high [Ca2+]) |
657(low [Ca2+]) 637(high [Ca2+]) |
Genacryl Brilliant Red B | 520 | 590 |
Genacryl Brilliant Yellow 10GF | 430 | 485 |
Genacryl Pink 3G | 470 | 583 |
Genacryl Yellow 5GF | 430 | 475 |
Gloxalic Acid | 405 | 460 |
Granular Blue | 355 | 425 |
Haematoporphyrin | 530-560 | 580 |
Hoechst 33258, 33342 (Bound to DNA) | 352 | 461 |
3-Hydroxypyrene-5,-8,10-TriSulfonic Acid | 403 | 513 |
7-Hydroxy-4-methylcoumarin | 360 | 455 |
5-Hydroxy-Tryptamine (5-HT) | 380-415 | 520-530 |
Indo-1 | 350 | 405-482 |
Intrawhite Cf Liquid | 360 | 430 |
Leucophor PAF | 370 | 430 |
Leucophor SF | 380 | 465 |
Leucophor WS | 395 | 465 |
Lissamine Rhodamine B200 (RD200) | 575 | 595 |
Lucifer Yellow CH | 425 | 528 |
Lucifer Yellow VS | 430 | 535 |
LysoSensor Blue DND-192, DND-167 | 374 | 425 |
LysoSensor Green DND-153, DND-189 | 442 | 505 |
LysoSensor Yellow/Blue | 384(low pH) 329(high pH) |
540(low pH) 440(high pH) |
LysoTracker Green | 504 | 511 |
LysoTracker Yellow | 534 | 551 |
LysoTracker Red | 577 | 592 |
Magdala Red | 524 | 600 |
Magnesium Green | 506 | 531 |
Magnesium Orange | 550 | 575 |
Maxilon Brilliant Flavin 10 GFF | 450 | 495 |
Maxilon Brilliant Flavin 8 GFF | 460 | 495 |
Mitotracker Green FM | 490 | 516 |
Mitotracker Orange CMTMRos | 551 | 576 |
MPS (Methyl Green Pyronine Stilbene) | 364 | 395 |
Mithramycin | 450 | 570 |
NBD | 465 | 535 |
NBD Amine | 450 | 530 |
Nile Red | 515-530 | 525-605 |
Nitrobenzoxadidole | 460-470 | 510-650 |
Noradrenaline | 340 | 490-520 |
Nuclear Fast Red | 289-530 | 580 |
Nuclear Yellow | 365 | 495 |
Nylosan Brilliant Flavin E8G | 460 | 510 |
Oregon Green 488 fluorophore | 496 | 524 |
Oregon Green 500 fluorophore | 503 | 522 |
Oregon Green 514 fluorophore | 511 | 530 |
Pararosaniline (Feulgen) | 570 | 625 |
Phorwite AR Solution | 360 | 430 |
Phorwite BKL | 370 | 430 |
Phorwite Rev | 380 | 430 |
Phorwite RPA | 375 | 430 |
Phosphine 3R | 465 | 565 |
Phosphine R | 480-565 | 578 |
Pontochrome Blue Black | 535-553 | 605 |
POPO-1, PO-PRO-1 | 434 | 456 |
Primuline | 410 | 550 |
Procion Yellow | 470 | 600 |
Propidium Iodide | 536 | 617 |
Pyronine | 410 | 540 |
Pyronine B | 540-590 | 560-650 |
Pyrozal Brilliant Flavin 7GF | 365 | 495 |
Quinacrine Mustard | 423 | 503 |
R-phycoerythrin | 565 | 575 |
Rhodamine 110 | 496 | 520 |
Rhodamine 123 | 511 | 534 |
Rhodamine 5 GLD | 470 | 565 |
Rhodamine 6G | 526 | 555 |
Rhodamine B | 540 | 625 |
Rhodamine B 200 | 523-557 | 595 |
Rhodamine B Extra | 550 | 605 |
Rhodamine BB | 540 | 580 |
Rhodamine BG | 540 | 572 |
Rhodamine Green fluorophore | 502 | 527 |
Rhodamine Red | 570 | 590 |
Rhodamine WT | 530 | 555 |
Rhodol Green fluorophore | 499 | 525 |
Rose Bengal | 540 | 550-600 |
Serotonin | 365 | 520-540 |
Sevron Brilliant Red 2B | 520 | 595 |
Sevron Brilliant Red 4G | 500 | 583 |
Sevron Brilliant Red B | 530 | 590 |
Sevron Orange | 440 | 530 |
Sevron Yellow L | 430 | 490 |
SITS (Primuline) | 395-425 | 450 |
SITS (Stilbene Isothiosulphonic Acid) | 365 | 460 |
Sodium Green | 507 | 535 |
Stilbene | 335 | 440 |
Snarf 1 | 563 | 639 |
Sulpho Rhodamine B Can C | 520 | 595 |
Sulpho Rhodamine G Extra | 470 | 570 |
SYTOX Green nucleic acid stain | 504 | 523 |
SYTO Green fluorescent nucleic acid stains | 494 ± 6 | 515 ± 7 |
515 ± 7 | 543 ± 13 | |
SYTO 17 red fluorescent nucleic acid stain | 621 | 634 |
Tetracycline | 390 | 560 |
TRITC (Tetramethyl Rhodamine Isothiocyanate) | 557 | 576 |
Texas Red | 596 | 615 |
Thiazine Red R | 510 | 580 |
Thioflavin S | 430 | 550 |
Thioflavin TCN | 350 | 460 |
Thioflavin 5 | 430 | 550 |
Thiolyte | 370-385 | 477-484 |
Thiozol Orange | 453 | 480 |
Tinopol CBS | 390 | 430 |
TOTO 1, TO-RRO-1 | 514 | 533 |
TOTO 3, TO-PRO-3 | 642 | 661 |
True Blue | 365 | 420-430 |
Ultralite | 656 | 678 |
Uranine B | 420 | 520 |
Uvitex SFC | 365 | 435 |
X-Rhodamine | 580 | 605 |
Xylene Orange | 546 | 580 |
XRITC | 582 | 601 |
YOYO-1, YOYO-PRO-1 | 491 | 509 |
YOYO-3, YOYO-PRO-3 | 612 | 631 |
Synonyms and Related Terms
Fluorochrom (Deut.); fluorescent stain; fluorescent dye
Resources and Citations
- Olympus LS: Fluorochrome table
- Richard C. Wolbers, Nanette T. Sterman, Chris Stavroudis, Notes for Workshop on New Methods in the Cleaning of Paintings, J.Paul Getty Trust, Los Angeles, 1990
- Random House, Webster's Encyclopedic Unabridged Dictionary of the English Language, Grammercy Book, New York, 1997
- The American Heritage Dictionary or Encarta, via Microsoft Bookshelf 98, Microsoft Corp., 1998
- Table of fluorochromes at http://pingu.salk.edu/flow/fluo.html